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| Status |
Public on Mar 23, 2021 |
| Title |
Cecr2 promotes somatic cell reprogramming as a downstream target of SALL4 [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Sall4 is among one of the most important reprogramming factors. However, the underline molecular mechanisms for such importance remains unclear. In this report, we first described the gene expression and chromatin accessibility dynamics in OKS-Sall4 induced somatic cell reprogramming, and screened a group of downstream targets of Sall4, among which, cecr2, regulated by Sall4 directly, can significantly promote OKS induced reprogramming. Moreover, Cecr2 and Sall4 shared several downstream targets, such as Esrrb, Nanog, T etc., at transcription level when overexpress in the context of OKS induced reprogramming. We further showed that the DTT domain of cecr2 was responding to the reprogramming activity. Our findings provide a new important reprogramming factors and suggesting the cecr2-associted protein network may contributes to overcoming the epigenetic barriers in reprogramming.
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| Overall design |
Examination of SALL4 binding in mouse embryonic stem cells.
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| Contributor(s) |
Wu L |
| Citation(s) |
33144328 |
| |
| Submission date |
Mar 28, 2020 |
| Last update date |
Mar 24, 2021 |
| Contact name |
Jing Liu |
| E-mail(s) |
liu_jing@gibh.ac.cn
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| Organization name |
Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
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| Department |
CAS Key Laboratory of Regenerative Biology
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| Lab |
Jing Liu's Lab
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| Street address |
190 Kai Yuan Avenue, Science Park,Guangzhou
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| City |
Guangzhou |
| State/province |
Guangdon |
| ZIP/Postal code |
510530 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE147681 |
Cecr2 promotes somatic cell reprogramming as a downstream target of SALL4 |
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| Relations |
| BioProject |
PRJNA616042 |
| SRA |
SRP254415 |
