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| Status |
Public on Jun 09, 2020 |
| Title |
The effects of expression of mutant ASXL1 and HHEX on hematopoietic cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To identify target genes of mutant ASXL1 (ASXL1-MT) and HHEX in hematopoietic cells, we performed RNA-seq using RUNX1-ETO expressing cord blood cells transduced with vector or ASXL1-MT together with vector or HHEX.
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| Overall design |
Method: RUNX1-ETO expressing cord blood cells were transduced with control vector, ASXL1-MT (coexpressing GFP), HHEX (coexpressing NGFR) or ASXL1-MT+HHEX. 48hr after trunsduction, GFP+/NGFR+ cells were sorted by FACS. Total RNA extracted from sorted GFP+/NGFR+ cells were then subjected to RNA-seq analysis.
Three biologically independent experiments were perfomed for each sample.
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| Contributor(s) |
Takeda R, Asada S, Matsumoto A, Yamasaki S, Shibata T, Goyama S, Kitamura T |
| Citation(s) |
32492700 |
| |
| Submission date |
Mar 27, 2020 |
| Last update date |
Jun 09, 2020 |
| Contact name |
Shuhei Asada |
| Organization name |
Institute of Medical Science, The University of Tokyo
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| Department |
Cellular Therapy
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| Lab |
Kitamura Lab
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| Street address |
4-6-1 Shirokanedai, Minato-ku
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| City |
Tokyo |
| ZIP/Postal code |
1088639 |
| Country |
Japan |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE147656 |
Role of HHEX in hematopoietic cells |
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| Relations |
| BioProject |
PRJNA615886 |
| SRA |
SRP254343 |
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