|
| Status |
Public on Jun 05, 2020 |
| Title |
Paired immunoglobulin-like receptors mediate innate memory to non-self MHC molecules |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Abstract: Immunological memory specific to previously encountered antigens is a cardinal feature of adaptive lymphoid cells. It is not known however whether innate myeloid cells retain memory of prior antigenic stimulation and respond to it more vigorously upon a second encounter. Here, we show that murine monocytes and macrophages acquire memory specific to MHC-I antigens and identify paired immunoglobulin-like receptors-A (PIR-A) as the MHC-I receptors necessary for the memory response. We demonstrate that deleting PIR-A in the recipient or blocking PIR-A binding to donor MHC-I molecules blocks memory and attenuates kidney and heart allograft rejection. Thus, innate myeloid cells acquire alloantigen-specific memory that can be targeted to improve transplant outcomes.
Data purpose: Targeting monocyte and macrophage receptors that detect MHC antigens blocks innate immune memory and attenuates transplant rejection
|
| |
|
| Overall design |
splenic monocytes from B6, BALB, C3H mice at 1wk and 4wk post immunization
|
| |
|
| Contributor(s) |
Lakkis FG, Abou-Daya K |
| Citation(s) |
32381589 |
| |
| Submission date |
Mar 26, 2020 |
| Last update date |
Jun 05, 2020 |
| Contact name |
Wei Chen |
| E-mail(s) |
wei.chen@chp.edu
|
| Organization name |
Children’s Hospital of Pittsburgh of UPMC
|
| Department |
Division of Pulmonary Medicine, Allergy and Immunology, Department of Pediatrics
|
| Street address |
4401 Penn Ave., Rangos Research Building, Room 9125
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15224 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (14)
|
| GSM4435026 |
splenic monocytes from B6 mice at 1wk post immunization rep1 |
| GSM4435027 |
splenic monocytes from B6 mice at 1wk post immunization rep2 |
| GSM4435028 |
splenic monocytes from BALB mice at 1wk post immunization rep1 |
| GSM4435029 |
splenic monocytes from BALB mice at 1wk post immunization rep2 |
| GSM4435030 |
splenic monocytes from C3H mice at 1wk post immunization rep1 |
| GSM4435031 |
splenic monocytes from C3H mice at 1wk post immunization rep2 |
| GSM4435032 |
splenic monocytes from B6 mice at 4wk post immunization rep1 |
| GSM4435033 |
splenic monocytes from B6 mice at 4wk post immunization rep2 |
| GSM4435034 |
splenic monocytes from B6 mice at 4wk post immunization rep3 |
| GSM4435035 |
splenic monocytes from BALB mice at 4wk post immunization rep1 |
| GSM4435036 |
splenic monocytes from BALB mice at 4wk post immunization rep2 |
| GSM4435037 |
splenic monocytes from BALB mice at 4wk post immunization rep3 |
| GSM4435038 |
splenic monocytes from C3H mice at 4wk post immunization rep1 |
| GSM4435039 |
splenic monocytes from C3H mice at 4wk post immunization rep2 |
|
| Relations |
| BioProject |
PRJNA615665 |
| SRA |
SRP254188 |
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