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| Status |
Public on Dec 31, 2020 |
| Title |
Identification of m6A residues at single nucleotide resolution using eCLIP and an accessible custom analysis pipeline |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have developed a modified eCLIP-based method (meCLIP) to identify m6A residues at single-nucleotide resolution. By coupling the improvements of eCLIP with an easy-to-use computational pipeline, we have successfully identified over 50,000 unique m6A residues in the two breast cancer cell lines that were analyzed (MCF-7 and MDA-MB-231) and over 8,000 unique residues in HEK-293 cells. We compared these residues to the sites called using the currently most utilized m6A identification method (miCLIP).
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| |
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| Overall design |
m6A identification in 3 cell lines (MCF-7, MDA-MB-231, and HEK-293) using modified eCLIP-based library preparation and custom analysis pipeline
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| |
|
| Contributor(s) |
Roberts JT, Johnson AM |
| Citation(s) |
33376190, 36441764 |
| |
| Submission date |
Mar 24, 2020 |
| Last update date |
Jan 04, 2023 |
| Contact name |
Justin Roberts |
| E-mail(s) |
jtroberts@southalabama.edu
|
| Organization name |
University of South Alabama
|
| Department |
Pharmacology
|
| Street address |
5851 USA Drive North, MSB 3370
|
| City |
Mobile |
| State/province |
AL |
| ZIP/Postal code |
36688 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (17)
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| Relations |
| BioProject |
PRJNA614917 |
| SRA |
SRP253857 |
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