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| Status |
Public on Mar 24, 2020 |
| Title |
Thymic B cells promote germinal center-like structures and the expansion of follicular helper T cells in lupus-prone mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by autoantibody production and immune complex deposition. Previous evidence showed abnormal accumulation of B cells in the thymus of lupus-prone mice, but the role of this population remains undefined. We analyzed the distribution, function, and properties of thymic B cells in the BWF1 murine model of SLE. We found that B cells proliferate and cluster in germinal center-like structures along with autoantibody-secreting cells in the thymus of diseased-BWF1 mice. These thymic B cells induced the differentiation of follicular helper T cells (TFH). Our data suggest that the accumulation of B cells in the thymus of BWF1 mice results in the formation of germinal center-like structures and the expansion of TFH cells, which may favor the differentiation of autoreactive plasma cells. Therefore, the thymus emerges as a niche supporting the maintenance of the pathogenic humoral response in the development of murine SLE.
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| Overall design |
Thymic B cells mRNA profiles of lupus-diseased-BWF1 mice (BWF1 mice) and non-lupus-susceptible mice (control mice) were generated by deep sequencing, in triplicate, using MiSeq Reagent Kit v3 150-cycle PE
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| Contributor(s) |
Hidalgo Y, Flores-Santibañez F, Dorner J, Martinez V |
| Citation(s) |
32411134 |
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| Submission date |
Mar 23, 2020 |
| Last update date |
May 18, 2020 |
| Contact name |
Yessia Hidalgo |
| E-mail(s) |
yhidalgo@ug.uchile.cl
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| Organization name |
Universidad de Chile
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| Department |
Sciences
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| Lab |
Immunology
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| Street address |
Las Palmeras 3425
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| City |
Santiago |
| State/province |
Ñuñoa |
| ZIP/Postal code |
7800003 |
| Country |
Chile |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA614005 |
| SRA |
SRP253666 |
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