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Series GSE147359 Query DataSets for GSE147359
Status Public on Mar 24, 2020
Title Thymic B cells promote germinal center-like structures and the expansion of follicular helper T cells in lupus-prone mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by autoantibody production and immune complex deposition. Previous evidence showed abnormal accumulation of B cells in the thymus of lupus-prone mice, but the role of this population remains undefined. We analyzed the distribution, function, and properties of thymic B cells in the BWF1 murine model of SLE. We found that B cells proliferate and cluster in germinal center-like structures along with autoantibody-secreting cells in the thymus of diseased-BWF1 mice. These thymic B cells induced the differentiation of follicular helper T cells (TFH). Our data suggest that the accumulation of B cells in the thymus of BWF1 mice results in the formation of germinal center-like structures and the expansion of TFH cells, which may favor the differentiation of autoreactive plasma cells. Therefore, the thymus emerges as a niche supporting the maintenance of the pathogenic humoral response in the development of murine SLE.
Overall design Thymic B cells mRNA profiles of lupus-diseased-BWF1 mice (BWF1 mice) and non-lupus-susceptible mice (control mice) were generated by deep sequencing, in triplicate, using MiSeq Reagent Kit v3 150-cycle PE
Contributor(s) Hidalgo Y, Flores-Santibañez F, Dorner J, Martinez V
Citation(s) 32411134
Submission date Mar 23, 2020
Last update date May 18, 2020
Contact name Yessia Hidalgo
Organization name Universidad de Chile
Department Sciences
Lab Immunology
Street address Las Palmeras 3425
City Santiago
State/province Ñuñoa
ZIP/Postal code 7800003
Country Chile
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (6)
GSM4427127 L1
GSM4427128 L2
GSM4427129 L3
BioProject PRJNA614005
SRA SRP253666

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Supplementary file Size Download File type/resource
GSE147359_RAW.tar 1.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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