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Series GSE147159 Query DataSets for GSE147159
Status Public on Oct 28, 2020
Title The role of NTS/NTSR1 signal in pancreatic cancer progression
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Pancreatic cancer is one of the uncurable cancers and 5-year survival rate is still under 10 %. Therefore, it is urgent to identify a new molecular target for cure. In this study, the mouse serial transplantations by pancreatic orthotopic inoculation model were utilized for screening of new targets. The gene expression of highly malignant cancer cell lines, established in our previous study, showed increased expression of neurotensin receptor-1 (NTSR1). Re-analysis of clinical databases revealed that its expression was higher in pancreatic cancer dependent upon its stage. Overexpression of NTSR1 in SUIT-2 and Panc-1 cells accelerated tumorigenic and metastatic ability in vivo. RNA-sequence analysis revealed that MAPK and NF-kB signaling pathway were activated with neurotensin (NTS) stimulation in highly malignant cancer cell lines from Panc-1 cells. Moreover, NTS induced not only MMP-9, but also CTGF and other pro-inflammatory cytokines and chemokines. Administration of SR48692, a selective antagonist for NTSR1, suppressed tumorigenicity in vivo. These data suggested that NTSR1 can be a diagnostic marker and a new molecular target for pancreatic cancer treatment.
Overall design Target genes for NTS in highly malignant Panc-1- cells were identified.
Contributor(s) Takahashi K, Ehata S, Miyauchi K, Morishita Y, Miyazawa K, Miyazono K
Citation(s) 33034134
Submission date Mar 18, 2020
Last update date Oct 28, 2020
Contact name Shogo Ehata
Organization name The University of Tokyo
Department Graduate School of Medicine
Lab Deaprtment of Molecular Pathology
Street address Hongo 7-3-1, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-0033
Country Japan
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (4)
GSM4419443 RNA-seq-Panc-1-3P2_no_treatment rep1
GSM4419444 RNA-seq-Panc-1-3P2_NTS rep1
GSM4419445 RNA-seq-Panc-1-3P2_no_treatment rep2
BioProject PRJNA613216
SRA SRP253185

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Supplementary file Size Download File type/resource
GSE147159_genes.fpkm_tracking.xlsx 4.0 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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