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| Status |
Public on May 17, 2020 |
| Title |
Single cell RNA sequencing analysis of in vitro DENV1 infected cells |
| Organisms |
Homo sapiens; Dengue virus 1 Nauru/West Pac/1974 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
CEM.NKR.DC-SIGN cells were infected in vitro with DENV1 (strain WestPac74) for 18 hours, after which either total viable cells or viable cells expressing surface DENV1 NS1 were isolated by flow cytometric sorting. Sorted cells were processed for scRNAseq analysis using either a standard Oligo(dT) primer, or an Oligo(dT) primer supplemented with a DENV-specific RT primer
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| |
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| Overall design |
Single cell RNA sequencing analysis of CEM.NKR.DC-SIGN cells infected with DENV1 (strain WestPac74)
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| |
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| Contributor(s) |
Waickman A, Currier J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 17, 2020 |
| Last update date |
May 17, 2020 |
| Contact name |
Adam Tully Waickman |
| Organization name |
SUNY Upstate Medical University
|
| Street address |
766 Irving Avenue
|
| City |
Syracuse |
| State/province |
New York |
| ZIP/Postal code |
13210 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL28282 |
Illumina NovaSeq 6000 (Dengue virus 1 Nauru/West Pac/1974; Homo sapiens) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE147110 |
Analysis of cell-associated DENV RNA by oligo(dT) primed 5' capture scRNAseq |
|
| Relations |
| BioProject |
PRJNA613040 |
| SRA |
SRP253084 |
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