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Series GSE147095 Query DataSets for GSE147095
Status Public on Sep 13, 2020
Title mTORC1 and B cell survival signal regulate formation of memory precursors in the germinal center [2]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary A still unanswered question is what drives the small fraction of activated germinal center (GC) B cells that survive to become long-lived quiescent memory B cells. We found here that a small population of light zone GC cells (CD38intBcl6hi/intEfnb1+) with lower mTORC1 activity favored the memory B cell fate. Constitutively high mTORC1 activity led to defects in formation of the CD38intBcl6hi/intEfnb1+ cells; conversely, decreasing mTORC1 activity resulted in relative enrichment of this memory-prone GC population over the recycling-prone one. Furthermore, the CD38intBcl6hi/intEfnb1+ cells had higher levels of Bcl2 and surface BCR, which in turn, contributed to their survival. Given the positive correlation between the strength of T cell help and mTORC1 activity, our data suggest a model in which weak help from T cells together with provision of an increased survival signal are key for GC B cells to assume a memory B cell fate.
Overall design mRNA profiles of GC and memory precursor B cells were generated by deep sequencing in duplicate or triplicate, using Illumina HiSeq 1500.
Contributor(s) Inoue T, Kawai C, Ohara O, Kurosaki T
Citation(s) 33045065
Submission date Mar 17, 2020
Last update date Nov 04, 2020
Contact name Takeshi Inoue
Organization name Osaka University
Street address 3-1 Yamadaoka
City Suita-shi
State/province Osaka
ZIP/Postal code 5650871
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (16)
GSM4416994 LZ#1
GSM4416995 LZ#2
GSM4416996 LZ#3
This SubSeries is part of SuperSeries:
GSE147096 mTORC1 and B cell survival signal regulate formation of memory precursors in the germinal center
BioProject PRJNA613156
SRA SRP253200

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE147095_pro-mem_RNA-seq.txt.gz 996.9 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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