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Series GSE147095 Query DataSets for GSE147095
Status Public on Sep 13, 2020
Title mTORC1 and B cell survival signal regulate formation of memory precursors in the germinal center [2]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary A still unanswered question is what drives the small fraction of activated germinal center (GC) B cells that survive to become long-lived quiescent memory B cells. We found here that a small population of light zone GC cells (CD38intBcl6hi/intEfnb1+) with lower mTORC1 activity favored the memory B cell fate. Constitutively high mTORC1 activity led to defects in formation of the CD38intBcl6hi/intEfnb1+ cells; conversely, decreasing mTORC1 activity resulted in relative enrichment of this memory-prone GC population over the recycling-prone one. Furthermore, the CD38intBcl6hi/intEfnb1+ cells had higher levels of Bcl2 and surface BCR, which in turn, contributed to their survival. Given the positive correlation between the strength of T cell help and mTORC1 activity, our data suggest a model in which weak help from T cells together with provision of an increased survival signal are key for GC B cells to assume a memory B cell fate.
 
Overall design mRNA profiles of GC and memory precursor B cells were generated by deep sequencing in duplicate or triplicate, using Illumina HiSeq 1500.
 
Contributor(s) Inoue T, Kawai C, Ohara O, Kurosaki T
Citation(s) 33045065
Submission date Mar 17, 2020
Last update date Nov 04, 2020
Contact name Takeshi Inoue
Organization name Osaka University
Street address 3-1 Yamadaoka
City Suita-shi
State/province Osaka
ZIP/Postal code 5650871
Country Japan
 
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (16)
GSM4416994 LZ#1
GSM4416995 LZ#2
GSM4416996 LZ#3
This SubSeries is part of SuperSeries:
GSE147096 mTORC1 and B cell survival signal regulate formation of memory precursors in the germinal center
Relations
BioProject PRJNA613156
SRA SRP253200

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE147095_pro-mem_RNA-seq.txt.gz 996.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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