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| Status |
Public on Jun 25, 2020 |
| Title |
Single-molecule regulatory architectures captured by chromatin fiber sequencing [Dm Fiber-seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
|
| Summary |
We report a method for precisely stenciling the structure of individual chromatin fibers onto their composite DNA templates using non-specific DNA N6-adenine methyltransferases. Single-molecule long-read sequencing using PacBio of these chromatin stencils enables nucleotide-resolution readout of the primary architecture of multi-kilobase chromatin fibers (Fiber-seq).
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| |
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| Overall design |
Fiber-seq, AdMTase-seq and m6A-IP-seq on Drosophila S2-DRSC cells. Fiber-seq and AdMTase-seq on human K562 cells. AdMTase-seq on human Hela cells.
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| |
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| Contributor(s) |
Stergachis A, Stamatoyannopoulos J |
| Citation(s) |
32587015 |
| |
| Submission date |
Mar 13, 2020 |
| Last update date |
Sep 24, 2020 |
| Contact name |
John A Stamatoyannopoulos |
| E-mail(s) |
jstam@altius.org
|
| Organization name |
Altius Institute / University of Washington
|
| Department |
Genome Sciences
|
| Lab |
Stamatoyannopoulos
|
| Street address |
2211 Elliott Avenue, 6th Floor
|
| City |
SEATTLE |
| State/province |
WA |
| ZIP/Postal code |
98121 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL28353 |
Sequel II (Drosophila melanogaster) |
| GPL28354 |
Sequel (Drosophila melanogaster) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE146942 |
Single-molecule regulatory architectures captured by chromatin fiber sequencing |
|
| Relations |
| BioProject |
PRJNA612475 |
| SRA |
SRP252715 |
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