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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 31, 2009 |
| Title |
Ctr9 Promoter Region Binding |
| Organism |
Mus musculus |
| Experiment type |
Genome variation profiling by genome tiling array
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| Summary |
To study the function of Paf1C in mouse ESCs, we generated an ES cell line stably expressing a location and affinity purification (LAP)-tagged Ctr9 fusiuon protein using the bacterial astificial chromosome (BAC)-based TransgeneOmics approach. To investigate whether pluripotency and lineage control genes differentially regulated upon Paf1C depletion are direct targets of the Paf1C, we analyzed the binding of the Ctr9-LAP fusion protein by ChIP-chip and identified 2175 promoter regions that were bound by Ctr9.
The supplementary bed file contains promoter regions bound by Ctr9.
Keywords: ChIP-chip
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| Overall design |
GFP-tagged Ctr9 ChIPed with GFP vs. mock in BAC-transgenic mouse ESCs.
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| Contributor(s) |
Ding L, Schulz H, Paszkowski-Rogacz M |
| Citation(s) |
19345177 |
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| Submission date |
Jan 30, 2009 |
| Last update date |
Oct 14, 2014 |
| Contact name |
Li Ding |
| E-mail(s) |
ding@mpi-cbg.de
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| Organization name |
MPI-CBG
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| Lab |
Buchholz Lab
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| Street address |
Pfotenhauerstr. 108
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| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
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| Platforms (1) |
| GPL5811 |
[Mm_PromPR] Affymetrix Mouse Promoter 1.0R Array |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA112339 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14654_RAW.tar |
195.6 Mb |
(http)(custom) |
TAR (of BED, CEL) |
| Processed data provided as supplementary file |
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