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| Status |
Public on Dec 07, 2021 |
| Title |
Gene expression profiling on heads of Drosophila chromodomain helicase DNA binding protein 1 (CHD1) deletion mutants |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
CHD1 is a conserved ATP-dependent chromatin remodeling factor with roles in transcriptional regulation and chromatin assembly. Here we show that deletion of Chd1 causes multiple behavioral and metabolic defects. In particular, it diminishes food-intake and results in shortened lifespan of mutant flies. These phenotypes can be rescued by brain-specific expression of Chd1 via the elav promoter. Consistently, neuron-specific expression of Chd1 rescues the wide-spread transcriptional dysregulation observed in the absence of CHD1.
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| Overall design |
RNA was prepared from heads of 10 day-old Drosophila females for RNA-seq analysis. Three genoytypes were used (Chd1-deficient, Chd1-rescued, elavGal4>UAS-Chd1)
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| Contributor(s) |
Schoberleitner I, Bauer I, Lusser A |
| Citation(s) |
34610319, 35983070 |
| |
| Submission date |
Mar 04, 2020 |
| Last update date |
Aug 25, 2022 |
| Contact name |
Ingo Bauer |
| Organization name |
Medical University of Innsbruck
|
| Department |
Institute of Molecular Biology
|
| Street address |
Innrain 80-82
|
| City |
Innsbruck |
| State/province |
Tirol |
| ZIP/Postal code |
6020 |
| Country |
Austria |
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| Platforms (1) |
| GPL26560 |
BGISEQ-500 (Drosophila melanogaster) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA610298 |
| SRA |
SRP251590 |
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