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| Status |
Public on Jun 30, 2022 |
| Title |
Single-cell RNA sequencing analysis of non-haematopoietic DNGR-1 lineage traced cells from murine spinal cord |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Stem cells are defined by two cardinal properties: limitless self-renewal and multipotency. We have serendipitously found that non-haematopoietic DNGR-1 lineage traced cells residing in the ependymal cell layer of the central nervous system display the two cardinal properties of stem cells, both in vitro and in vivo. However, whether these properties were a feature of all DNGR-1-traced cells or were confined to a particular subset of these is unclear. To address the potential heterogeneity of DNGR-1-traced ependymal cells and caractherise their putative stem cell compartment we conducted single-cell RNA sequencing of DNGR-1-traced cells isolated from uninjured spinal cords.
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| Overall design |
DNGR-1 lineage traced cells pooled from a spinal cords dissected from DNGR-1 lineage tracer mice were FACS sorted and subjected to single-cell RNA sequencing
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| Contributor(s) |
Frederico B, Reis e Sousa C, Chakravarty P |
| Citation(s) |
35998585 |
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| Submission date |
Mar 02, 2020 |
| Last update date |
Sep 29, 2022 |
| Contact name |
Bruno Frederico |
| E-mail(s) |
frederico.bruno@gmail.com
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| Organization name |
Francis Crick Institute
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| Street address |
1 Midland Road
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| City |
London |
| ZIP/Postal code |
NW1 1AT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA609742 |
| SRA |
SRP251282 |
