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Series GSE146145 Query DataSets for GSE146145
Status Public on Jan 27, 2021
Title Pathways involved in pony body size development
Organism Equus caballus
Experiment type Expression profiling by high throughput sequencing
Summary The mechanism of body growth in mammals is poorly understood. Here, we report the regulatory networks involved in body growth through analyzing transcriptomes of pituitary and epiphyseal tissues of Debao ponies and Mongolian horses at juvenile and adult stages. We found that GHR was expressed little in long bones though GH was highly expressed in Debao ponies compared with Mongolian horses. Moreover, m-RAS and ATF3, involved in the GHR pathway, were found to be significantly downregulated in juvenile ponies, which slowed the proliferation of bone osteocytes. However, WNT2 and PLCβ2 were obviously upregulated in juvenile Debao ponies, which led to premature mineralization of bone extracellular matrix. Furthermore, we found that the WNT/Ca2+ pathway may be responsible for the regulation of body growth. We then demonstrated that GHR was lacking in long bones of Debao ponies using RT-qPCR and Western blot. Treatment with WNT antagonist 1 decreased expression of the WNT pathway (P≤0.05) in vitro. The transduction of ATDC5 cells with GHR-RNAi lentivirus decreased expression of the GHR pathway (P≤0.05). Additionally, detection of plasma hormone concentrations showed that the ponies had higher levels of IGF-1 as juveniles and GH in adulthood than Mongolian horses, indicating that the hormone regulation in Debao ponies differs from that in Mongolian horses. Our work provides an insight into the genetic regulation for dwarf growth in mammals and a reference for therapeutic strategy for dwarfism
Overall design Twenty-four samples were taken and prepared from Six female DPs and six female MHs pituitary and epiphysis tissues.
Citation(s) 33461495
Submission date Mar 01, 2020
Last update date Jan 27, 2021
Contact name Dong Chen
Organization name ABLife, Inc.
Department Center for Genome Analysis
Street address 388 GaoXin 2nd Road, East Lake Hi-Tech Development Zone
City Wuhan
State/province Hubei
ZIP/Postal code 430075
Country China
Platforms (1)
GPL21401 Illumina NextSeq 500 (Equus caballus)
Samples (24)
GSM4367188 DPLBA1: adult Debao pony long bone1
GSM4367189 DPLBA2: adult Debao pony long bone2
GSM4367190 DPLBA3: adult Debao pony long bone3
BioProject PRJNA609553
SRA SRP251177

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE146145_expressed_gene_RPKM.txt.gz 1.3 Mb (ftp)(http) TXT
GSE146145_expressed_gene_reads.txt.gz 1.1 Mb (ftp)(http) TXT
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Processed data are available on Series record

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