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Series GSE146123 Query DataSets for GSE146123
Status Public on Feb 29, 2020
Title Dental cell type atlas reveals new stem and differentiated cell types in mouse and human teeth
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Reconstruction and engineering of human teeth are the tasks of utmost importance. Here we introduce detailed cellular atlas of the growing and non-growing mouse and human teeth including a condition with caries, which will enhance the field of dental engineering and lead to a better understanding of stem cell niche dynamics enabling tooth growth. We report previously unappreciated cellular complexity of the mouse incisor, including new spatially-restricted stem, progenitor and differentiated populations and compare this model system with the human adult, growing and caries-lesioned teeth. This comparison revealed specific parallelisms in mechanisms of dental growth in humans and mice. Beyond the main mesenchymal and epithelial compartments, we demonstrate a surprising role of the immune system as a mediator of the epithelial-mesenchymal interactions required for proper development. Finally, our results brought new insights into the differentiation of cell lineages producing enamel and dentin, which should be of utmost importance for dental reconstructive engineering.
Overall design Analysis of cellular dynamics during homeostasis and maturation in human and mouse teeth using single-cell expression profiling

Please note that counts_SS2_16_257.txt (linked to the GSM4365617 records) was generated from both 'S2_16_257.PLP/YFP 3 days traced' and 'SS2_16_257.whole' samples, and the counts_SS2_17_014.txt (linked to the GSM4365621 records) from both 'SS2_17_014.Col2.3+' and 'SS2_17_014.DMP1+' samples.
Contributor(s) Krivanek J, Soldatov R, Kharchenko PV, Adameyko I
Citation(s) 32968047
Submission date Feb 28, 2020
Last update date Oct 13, 2020
Contact name Ruslan Soldatov
Organization name Harvard Medical School
Department Biomedical Informatics
Lab Kharchenko
Street address 10 Shattuck St.
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (3)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (24)
GSM4365601 human_germinectomy.apical papilla
GSM4365602 human_molar_pulp.whole
GSM4365603 MF183_Dent.whole
BioProject PRJNA609340
SRA SRP251055

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE146123_RAW.tar 98.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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