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Series GSE145959 Query DataSets for GSE145959
Status Public on Feb 25, 2021
Title microarray Analysis of MKN-45 cell lines after 48 hours post-transfection of miR-125b mimic (50nM) and mimic control.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We demonstrate that miR-125b, a key node in this microRNA regulatory network, is upregulated in gastric cancer (GC) and associated with poor overall survival through an integrated analysis of microRNA and mRNA profiling of GC revealed a mRNA-regulatory network.So we have employed whole genome microarray expression profiling as a discovery platform to compare the transcriptome profiling of human gastric cells (MKN-45) after 48 hours post-transfection of miR-125b mimic (50nM) and mimic control.Pathway analysis shows that the predicted targets of miR-125b are highly involved in apoptosis/program death pathway,and the robust apoptosis genes, BIK and CASP6 are validated as the directed targets of miR-125b.
Overall design Gene expression profiling was examined by the Agilent platform (AgilentG3-_GX_1color) with MKN-45 cells and analysis with One-Color Microarray-Based Gene Expression Analysis protocol (Agilent). At 48 hours post-transfection of miR-125b mimic and mimic control, total RNAs were isolated from cells by Trizol reagents. The integrity of total RNAs was evaluated by an Agilent 2100 Bioanalyzer (Agilent). Cy3-labeled target cRNA was prepared by Low Input Quick Amp Labeling Kit (Agilent) according to the manufacturer’s instructions. Labeled cRNAs were hybridized with a SurePrint G3 Human GE 8×60K Microarrays (Agilent).
Contributor(s) Liu B
Citation(s) 33947843
Submission date Feb 26, 2020
Last update date May 28, 2021
Contact name ben liu
Phone 86-2223372231
Organization name Tianjin Medical University Cancer Institute and Hospital
Department Department of Epidemiology and Biostatistics
Street address Huanhuxi Road
City Tianjin
ZIP/Postal code 300000
Country China
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (2)
GSM4340187 MKN-125b_48h_control
GSM4340188 MKN-125b_48h_mimic
BioProject PRJNA608875

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE145959_RAW.tar 11.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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