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Series GSE145922 Query DataSets for GSE145922
Status Public on May 14, 2020
Title IL-23 promotes a coordinated B-cell germinal center program for class-switch recombination to IgG2b in BXD2 mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary IL-23 promotes autoimmune disease including Th17 CD4 T-cell development and autoantibody (autoAb) production. Here, we show that a deficiency of the p19 component of IL-23 in autoimmune BXD2 (BXD2-p19−/−) mice leads to an imbalance of the follicular T-helper cell program with a shift from Tfh-IL-17 to Tfh-IFN-ɣ. Although the germinal center (GC) size and number of GC B cells remained the same, BXD2-p19−/− mice exhibited a lower class-switch recombination (CSR) program in the GC B cells, leading to a lower serum levels of IgG2b. Single cell transcriptomics analysis revealed that while GC B cell expression of Ifngr1 and Il21r genes exhibited a synchronized expression pattern with plasma cell program genes, Il17ra exhibited a synchronized expression pattern with pre-plasmablast GC program genes. Down-regulation of Ighg2b in BXD2-p19−/− GC B cells was associated with decreased expression of CSR-related base excision repair genes that were otherwise predominantly expressed by Il17ra+ cells compared to Ifngr1+ GC B cells in BXD2 mice. Together, these results suggest that although IL-23 is dispensable for GC formation, it is essential for development of the Tfh-IL17 Tfh subpopulation, which drives CSR-related base excision repair genes during the pre-plasmablast GC stage of development.
Overall design We carried out single cell RNAseq and single-cell V(D)J seq for GC B cells derived from the spleens of BXD2 mice and IL-23-deficient BXD2 (BXD2-p19-/-)mice
Contributor(s) Hong H, Gao M, Liu S, Hsu H, Mountz JD
Citation(s) 32554431
Submission date Feb 25, 2020
Last update date Jun 09, 2021
Contact name Min Gao
Organization name University of Alabama at Birmingham
Street address 1900 University Blvd, THT Building Suite #130G
City Birmingham
State/province AL
ZIP/Postal code 35294
Country USA
Platforms (2)
GPL16417 Illumina MiSeq (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (10)
GSM4339439 BXD2-p19-/-_#1_single_cell_RNAseq
GSM4339440 BXD2-p19-/-_#2_single_cell_RNAseq
GSM4339441 BXD2-p19-/-_#3_single_cell_RNAseq
BioProject PRJNA608738
SRA SRP250728

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Supplementary file Size Download File type/resource
GSE145922_RAW.tar 75.4 Mb (http)(custom) TAR (of CSV, MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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