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| Status |
Public on Jun 30, 2022 |
| Title |
Bulk transcriptomic comparison between in vitro propagated DNGR-1-lineage traced cells and hippocampus derived neural stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We compared by bulk RNA sequencing the transcriptomic profile of in vitro cultured DNGR-1-traced neural stem cells or their differentiated astrocytic progeny with similar cell fates generated from bonafide neural stem cells derived from the hippocampus.
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| Overall design |
Passage 10 NSCs adherent cultures were generated from tdTomato+, EYFP+ sorted from neurospheres grown from spinal cords of DNGR-1CreROSALSLtdTomato ot DNGR-1CreROSALFLEYFP mice, respectively. Hippocampus derived adherent NSCs cultures were generated from the hippocampus of GlastCreHuweflROSALSLEYFP mice. NSCs seeded in adherent conditions (+eGFP +bFGF+laminin) were recovered in StemPro accutase after 4 days or induced to differentiate into astrocytes (+BMP4) for 6 days. Cells from either condition were lysed with RLT buffer supplemented with 2-mercaptoethanol and cells lysates stored at -80C. Three replicates for each cell fate (3x10_6 NSCs or astrocytes) generated from passages 10, 11 and 12 were sequenced..
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| Contributor(s) |
Frederico B, Reis e Sousa C, Chakravarty P |
| Citation(s) |
35998585 |
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| Submission date |
Feb 24, 2020 |
| Last update date |
Sep 29, 2022 |
| Contact name |
Bruno Frederico |
| E-mail(s) |
frederico.bruno@gmail.com
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| Organization name |
Francis Crick Institute
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| Street address |
1 Midland Road
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| City |
London |
| ZIP/Postal code |
NW1 1AT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA608461 |
| SRA |
SRP250535 |
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