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Series GSE145777 Query DataSets for GSE145777
Status Public on Mar 22, 2021
Title Mapping H3K27ac in human MAIT cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Mucosal associated invariant T (MAIT) cells, already differentiated and located at mucosal sites, are critical in the body’s first wave of defenses against invading pathogens. Bcl11b KO MAIT cells fail to be maintained both in the thymus and peripheral organs. Furthermore, MAIT cells fail to fully develop in the thymus without Bcl11b, failing to upregulate RORγt, and that phenotype remains in the lungs and livers of these mice. Bcl11b deletion in MAIT cells causes dramatic shifts in the activation and TH17 programs, due to the binding of Bcl11b in many of those genes, which we have seen in the human MAIT cells. MAIT cells rely on PLZF and RORγt for their development and function, while also heavily relying on Bcl11b. These data show the key interplay of Bcl11b with PLZF and RORγt in a T cell leading to its development and necessary function to protect the body against diseases.
Overall design Examination of H3K27ac in human MAIT cells from peripheral blood
Contributor(s) Drashansky T, Helm E, Huo Z, Avram D
Citation(s) 33870128
Submission date Feb 23, 2020
Last update date May 25, 2021
Contact name Dorina Avram
Organization name Moffitt Cancer Center
Department Department of Immunology
Lab Avram lab
Street address 12902 Magnolia Drive, MRC4
City Tampa
State/province Fl
ZIP/Postal code 33612
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (4)
GSM4332643 Human_H3K27ac_MAIT_1
GSM4332644 Human_H3K27ac_MAIT_2
GSM4332645 Human_H3K27ac_Input_1
This SubSeries is part of SuperSeries:
GSE145779 Bcl11b binding and transcriptome analysis of Bcl11b-deficient MAIT cells at steady state and infection
BioProject PRJNA608198
SRA SRP250434

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Supplementary file Size Download File type/resource
GSE145777_RAW.tar 1.1 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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