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Series GSE145743 Query DataSets for GSE145743
Status Public on Aug 01, 2020
Title Genome-wide CRISPR knockout screen using GeCKO v2 to identify synthetic lethal interactions with the PARP inhibitor Olaparib
Organisms Homo sapiens; synthetic construct
Experiment type Other
Summary In this study, a population of knockout cells was generated using the GeCKO v2 sgRNA library. The population of knockout cells was treated with the PARP inhibitor olaparib for 14 days. Over this time, knockout cells that were sensitive to olaparib treatment would die and a population of knockout cells that was resistant to olaparib treatment remained. Cells from olaparib and DMSO conditions and the sgRNA cassette in the knockout cells was amplified and sequenced to identify synthetic lethal or suppressor interactions with olaparib treatment.
 
Overall design CRISPR screen using Human GeCKOv2 Library A or B comparing Olaparib and DMSO (control) treated Hela cells in 2 biological replicates.
 
Contributor(s) Smith R, Schauer T, Timinszky G
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Submission date Feb 21, 2020
Last update date Aug 07, 2020
Contact name Tamas Schauer
E-mail(s) tamas.schauer@helmholtz-munich.de
Organization name Helmholtz Zentrum München
Department Institute of Epigenetics and Stem Cells
Street address Feodor-Lynen-Straße 21
City Munich
ZIP/Postal code 81377
Country Germany
 
Platforms (2)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
GPL28978 Illumina HiSeq 1500 (synthetic construct)
Samples (12)
GSM4332020 GeCKO-A input control, Day 0
GSM4332021 GeCKO-A_DMSO-2, Day 14
GSM4332022 GeCKO-A_Olaparib-1, Day 14
Relations
BioProject PRJNA608032
SRA SRP250346

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE145743_my_counts_anno_merged.txt.gz 1.5 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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