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Series GSE145729 Query DataSets for GSE145729
Status Public on Feb 13, 2023
Title Estrogen-induced CircRNA, CircPGR, Functions as a CeRNA to Promote Estrogen Receptor-positive Breast Cancer Progression by Regulating Cell Cycle-related Genes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Estrogen and estrogen receptor (ER)-mediated gene transcriptional events have been well known to be involved in ER-positive breast carcinogenesis. Meanwhile, circular RNAs (circRNAs) are emerging as a new family of functional non-coding RNAs (ncRNAs) with implications in a variety of pathological processes, such as cancer. However, the estrogen-regulated circRNA program and the function of such program remain uncharacterized. Here, genome-wide circRNA profiling by circRNA sequencing (circRNA-seq) revealed tens of thousands of circRNAs were induced by estrogen, and further functional screening for the several circRNAs originated from PGR revealed that one of them, which we named as circPGR, was required for ER-positive breast cancer cell growth and tumorigenesis. CircPGR was found to be localized in the cytosol of cells. Mechanistically, circPGR functioned as a competing endogenous RNA (ceRNA) to sponge miR-301a-5p to regulate the expression of multiple cell cycle genes. The clinical relevance of circPGR was underscored by its high and specific expression in ER-positive breast cancer cell lines and clinical breast cancer tissue samples. Accordingly, siRNA targeting circPGR was proven to be effective in suppressing ER-positive breast cancer cell growth. These findings reveled that, besides the well-known mRNA, microRNA (miRNA), long non-coding RNA (lncRNA) and enhancer RNA (eRNA) programs, estrogen also induced a circRNA program, and exemplified by circPGR, these estrogen-induced circRNAs were required for ER-positive breast cancer cell growth, providing a new class of therapeutic targets for ER-positive breast cancer.
Overall design CircRNA-seq performed in this study was designed to uncover the estrogen-induced circRNA program in MCF7 cells; RNA-seq performed in this study was designed to understand the molecular mechanisms underlying circPGR in gene transcriptional activation.
Contributor(s) Liu W, Wang L, Hu G, Liu YC
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Submission date Feb 21, 2020
Last update date Feb 15, 2023
Contact name Guosheng Hu
Phone 15506972603
Organization name xiamen University
Department School of Pharmaceutical Sciences
Lab liulab
Street address Xiang'an South Road, Xiang'an District
City Xiamen
State/province Fujian
ZIP/Postal code 361102
Country China
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (4)
GSM4331622 circRNA-Seq_MCF7_DMSO
GSM4331623 circRNA-Seq_MCF7_E2
GSM4331624 RNA-Seq_MCF7_siCTL_DMSO
BioProject PRJNA608011
SRA SRP250326

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE145729_RAW.tar 314.2 Mb (http)(custom) TAR (of BIGWIG, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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