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Series GSE145599 Query DataSets for GSE145599
Status Public on Mar 04, 2020
Title The function and mechanism about how ORAOV1-B potentiates the OSCC metastasis.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Lymph node metastasis, a powerful prognostic indicator of oral squamous cell carcinoma (OSCC), is chiefly responsible for cancer death. Long non-coding RNA (lncRNA) is recently addressed to significantly account for modulating OSCC metastasis. Here, we identified a novel alternative splice of Oral Cancer Overexpressed 1 (ORAOV1), ORAOV1-B, which was subsequently validated as an lncRNA and correlated with OSCC lymph node metastasis; significantly increased invasion and migration were observed in ORAOV1-B-overexpressed OSCC cells; In the metastatic model, ORAOV1-B also significantly contributed to OSCC-related lung metastasis. cDNA microarrays was performed to compare up- or down-regulated genes in EV and ORAOV1-B sets of OSCC, which suggested TNFα as a potential downstream of ORAOV1-B and the upregulation of pro-inflammatory genes, indicating the activation of NF-κB pathway.
In summary, the novel splice variant ORAOV1-B is an lncRNA, which significantly potentiates OSCC invasion and metastasis by binding to Hsp90 and activating the NF-κB-TNFα loop. Our study implies that ORAOV1-B might serve as an attractive OSCC metastasis intervention target.
Overall design OSCC tissue study showed that ORAOV1-B was significantly higher in OSCC patients with lymph node metastasis. Overexpression of ORAOV1-B by lentivirus in OSCC cells could verify the function and mechanism of ORAOV1-B in OSCC progression. HSC-3 and HSC-4 cells were transduced with EV or ORAOV1-B lentivirus to generate the stably cells with overexpressed ORAOV1-B or not. cDNA microarray was performed on EV or ORAOV1-B sets of HSC-3 and HSC-4 cells to detect some ORAOV1-B-regulating pathways or genes involved in the process of tumor invasion and metastasis.
Contributor(s) Luo X, Chen F, Jiang Y, Qiu Y, Xu H, Wei Z, Tian G, Gong W, Yuan Y, Feng H, Zhong L, Ji N, Xu X, Sun C, Li T, Li J, Feng X, Deng P, Zeng X, Zhou M, Zhou Y, Dan H, Jiang L, Chen Q
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Submission date Feb 20, 2020
Last update date Mar 06, 2020
Contact name Qianming Chen
Organization name Sichuan University
Department oral medicine
Street address Renmin Nan Street Section 3 NO.14
City Chengdu
State/province Sichuan
ZIP/Postal code 610041
Country China
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (4)
GSM4322067 HSC-3 EV
GSM4322068 HSC-3 ORAOV1-B overexpression
GSM4322069 HSC-4 EV
BioProject PRJNA609923

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Supplementary file Size Download File type/resource
GSE145599_RAW.tar 12.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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