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| Status |
Public on May 28, 2020 |
| Title |
RNA-seq of murine megakaryocyte progenitor cells treated with LSD1 inhibitor INCB059872 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
INCB059872 is a selective irreversible inhibitor of Lysine-Specific Demethylase 1 (LSD1) that is in phase 1 clinical trials in hematopoietic malignancies. We identified a population of cells within murine lineage-negative bone marrow that is expanded after treatment with INCB059872. Here we sorted cells from this population (Lin- Cd41+ Cd200r3-) and performed RNA-seq to measure gene expression changes caused by INCB059872. Consistent with the thrombocytopenia observed in LSD1i-treated mice, INCB059872 reduced expression of genes associated with platelet function.
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| Overall design |
Mice were treated for 0, 4, or 6 days with 10 mg/kg INCB059872 (2 mice per treatment group) before harvesting bone marrow and sorting megakaryocyte progenitor cells for RNA-seq.
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| Contributor(s) |
Johnston G, Hiebert S |
| Citation(s) |
32422235 |
| |
| Submission date |
Feb 12, 2020 |
| Last update date |
May 28, 2020 |
| Contact name |
Scott Hiebert |
| Organization name |
Vanderbilt University
|
| Street address |
2220 Pierce Ave.
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA606326 |
| SRA |
SRP248473 |
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