GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE145107 Query DataSets for GSE145107
Status Public on Mar 07, 2020
Title Gonadotropin surge-induced expression of progesterone receptor serves the ovary as a trigger of ovulation and a terminator of ovulatory inflammation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Ovulation is triggered by the gonadotropin surge that induces the expression of two key genes, progesterone receptor (Pgr) and prostaglandin-endoperoxide synthase 2 (Ptgs2) in the granulosa cells of preovulatory follicles. Their gene products PGR and PTGS2 activate two separate pathways that are both essential for successful ovulation. Here we show that the PGR plays an additional essential role; attenuate ovulatory inflammation by diminishing the gonadotropin surge-induced Ptgs2 expression. PGR indirectly terminates Ptgs2 expression and PGE2 synthesis in granulosa cells by inhibiting the NF-κB, a transcription factor required for Ptgs2 expression. When the expression of PGR was ablated in the granulosa cells, the ovary undergoes hyperinflammatory condition manifested by excessive PGE2 synthesis, immune cell infiltration, oxidative damage, and neoplastic transformation of ovarian cells. Despite the ovary undergoes ovulations dozens or hundreds of times in one’s lifetime, the repetitive ovulatory inflammations do not leave significant tissue damage in the ovary. The PGR-driven termination of PTGS2 expression may protect ovary from the ovulatory inflammation.
Overall design Wild type (Pgr flox/flox) and Esr2-driven Pgr conditional knockout (Esr2 iCre/wt Pgr flox/flox) mice were injected with PMSG at the age of 25 days after birth. On day 27, mice were injected with hCG to induce the superovulation. At 6h after hCG injection, 8 ovaries were collected from 4-different mice in each group and single-cell mRNA libraries were generated by Single-Cell 3’ Chromium kit version 3 from 10X Genomics.
Contributor(s) Park CJ, Lin P, Zhou S, Barakat R, Bashir ST, Choi JM, Cacioppo JA, Oakley OR, Duffy DM, Lydon JP, Ko CJ
Citation(s) 32294429
NIH grant(s)
Grant ID Grant title Affiliation Name
P01 HD071875 Mechanism of Periovulatory Leukocyte Infiltration into the Ovary UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION CHEMYONG Jay KO
R21 HD094296 Conversion of ERalpha cells to ERbeta cells in a cell lineage UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN CHEMYONG Jay KO
Submission date Feb 11, 2020
Last update date May 18, 2020
Contact name CheMyong Jay Ko
Organization name University of Illinois at Urbana Champaign
Department Comparative Biosciences
Street address 2001 S. Lincoln Ave.
City Urbana
State/province Illinois
ZIP/Postal code 61802
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM4306638 ovary, WT_hCG6h
GSM4306639 ovary, KO_hCG6h
BioProject PRJNA606048
SRA SRP248225

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE145107_RAW.tar 107.5 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap