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Series GSE145071 Query DataSets for GSE145071
Status Public on May 28, 2020
Title PRO-seq of THP-1 cells treated with LSD1 inhibitor INCB059872
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Other
Summary INCB059872 is a selective irreversible inhibitor of Lysine-Specific Demethylase 1 (LSD1) that is in phase 1 clinical trials in hematopoietic malignancies. We used precision nuclear run-on sequencing (PRO-seq) to define early transcriptional changes associated with INCB059872 treatment. Changes in nascent transcription could be traced back to a loss of CoREST activity resulting in activation of GFI1-regulated targets.
 
Overall design THP-1 cells were treated (in duplicate) with 25nM INCB059872 for 0, 6, 12, or 24hrs. PRO-seq was used to measure changes in nascent transcription.
 
Contributor(s) Johnston G, Hiebert S
Citation(s) 32422235
Submission date Feb 10, 2020
Last update date May 30, 2020
Contact name Scott Hiebert
Organization name Vanderbilt University
Street address 2220 Pierce Ave.
City Nashville
State/province TN
ZIP/Postal code 37232
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (8)
GSM4305745 THP-1 cells, control A
GSM4305746 THP-1 cells, control B
GSM4305747 THP-1 cells, 24hr INCB059872 A
Relations
BioProject PRJNA605859
SRA SRP248057

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Supplementary file Size Download File type/resource
GSE145071_RAW.tar 1.1 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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