GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE144716 Query DataSets for GSE144716
Status Public on Apr 20, 2022
Title Gene regulation by gonadal hormone receptors defines brain sex differences-[CUT&RUN]
Organisms Homo sapiens; Mus musculus
Experiment type Other
Summary Estradiol establishes neural sex differences in many vertebrates and modulates mood, behavior, and energy balance in adulthood. In the canonical pathway, estradiol exerts its effects through the transcription factor estrogen receptor α (ERα). While ERα has been extensively characterized in breast cancer, the neuronal targets of ERα, and their involvement in brain sex differences, remain largely unknown. Here we generate a comprehensive map of genomic ERα-binding sites within a sexually dimorphic neural circuit that mediates social behaviors. We conclude that ERα orchestrates sexual differentiation of the mouse brain through two mechanisms: establishing two male-biased neuron types and activating a sustained male-biased gene expression program. Collectively, our findings reveal that sex differences in gene expression are defined by hormonal activation of neuronal steroid receptors. The molecular targets we identify may underlie the effects of estradiol on brain development, behavior, and disease.
Overall design Genomic response to estradiol in mouse brain
Contributor(s) Gegenhuber B, Wu M, Bronstein R, Tollkuhn J
Citation(s) 35508660
NIH grant(s)
Grant ID Grant title Affiliation Name
F31 MH124365 Estrogen receptor regulation of brain sexual differentiation COLD SPRING HARBOR LABORATORY Jordan Bruno Gegenhuber
R01 MH113628 Epigenetic regulation of sex differences in the brain COLD SPRING HARBOR LABORATORY Jessica Tollkuhn
Submission date Feb 03, 2020
Last update date May 09, 2022
Contact name Melody Wu
Organization name Cold Spring Harbor Laboratory
Street address 1 Bungtown Rd
City Cold Spring Harbor
ZIP/Postal code 11724
Country USA
Platforms (3)
GPL16417 Illumina MiSeq (Mus musculus)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (34)
GSM4294279 IgG_CR_veh
GSM4294280 IgG_CR_E2
GSM4294281 MCF7_ERa_Ab1_veh_CR_1
This SubSeries is part of SuperSeries:
GSE144718 Gene regulation by gonadal hormone receptors defines brain sex differences
BioProject PRJNA604630
SRA SRP247002

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE144716_RAW.tar 2.6 Gb (http)(custom) TAR (of BW, NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap