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| Status |
Public on Apr 20, 2022 |
| Title |
Gene regulation by gonadal hormone receptors defines brain sex differences-[CUT&RUN] |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Other
|
| Summary |
Estradiol establishes neural sex differences in many vertebrates and modulates mood, behavior, and energy balance in adulthood. In the canonical pathway, estradiol exerts its effects through the transcription factor estrogen receptor α (ERα). While ERα has been extensively characterized in breast cancer, the neuronal targets of ERα, and their involvement in brain sex differences, remain largely unknown. Here we generate a comprehensive map of genomic ERα-binding sites within a sexually dimorphic neural circuit that mediates social behaviors. We conclude that ERα orchestrates sexual differentiation of the mouse brain through two mechanisms: establishing two male-biased neuron types and activating a sustained male-biased gene expression program. Collectively, our findings reveal that sex differences in gene expression are defined by hormonal activation of neuronal steroid receptors. The molecular targets we identify may underlie the effects of estradiol on brain development, behavior, and disease.
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| Overall design |
Genomic response to estradiol in mouse brain
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| Contributor(s) |
Gegenhuber B, Wu M, Bronstein R, Tollkuhn J |
| Citation(s) |
35508660 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| F31 MH124365 |
Estrogen receptor regulation of brain sexual differentiation |
COLD SPRING HARBOR LABORATORY |
Jordan Bruno Gegenhuber |
| R01 MH113628 |
Epigenetic regulation of sex differences in the brain |
COLD SPRING HARBOR LABORATORY |
Jessica Tollkuhn |
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| |
| Submission date |
Feb 03, 2020 |
| Last update date |
May 09, 2022 |
| Contact name |
Melody Wu |
| E-mail(s) |
mwu@cshl.edu
|
| Organization name |
Cold Spring Harbor Laboratory
|
| Street address |
1 Bungtown Rd
|
| City |
Cold Spring Harbor |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (34)
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| This SubSeries is part of SuperSeries: |
| GSE144718 |
Gene regulation by gonadal hormone receptors defines brain sex differences |
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| Relations |
| BioProject |
PRJNA604630 |
| SRA |
SRP247002 |
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