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Series GSE144702

Query DataSets for GSE144702

Status

Public on Feb 04, 2020

Title

Distinct signature, origin and dynamics of macrophages in the peripheral and central nervous system (microarray)

Organism

Experiment type

Expression profiling by array
Third-party reanalysis

Summary

We performed ontogenic, transcriptomic and spatial characterization of sciatic nerve Macs (snMacs). Using multiple fate-mapping systems, we show that snMacs do not derive from the early embryonic precursors colonizing the CNS, but originate primarily from late embryonic precursors and get replaced by bone marrow-derived Macs over time. Using single-cell profiling, we identified a tissue-specific core signature of snMacs and found two spatially-separated snMacs: Relmα + Mgl1 + snMacs in the epineurium and Relmα Mgl1 snMacs in the endoneurium. Globally, snMacs lack most core signature genes of microglia, with only the endoneurial subset expressing a restricted number of these genes. Single-cell transcriptomics revealed that in response to injury both snMacs respond differently and that the PNS, in contrast to the CNS, is permissive to prolonged engraftment of monocyte-derived Macs recruited upon injury.

Overall design

Microarray = Sciatic Nerve macrophages in steady state and different time points (1.5, 5, 10 and 37 days) after nerve crush. This data was compared to previously published data (GSE75225 and GSE117080) and to other tissue resident macrophages from the Immgen Consortium Database (GSE15907).
Bulk RNA Seq = Brain microglia, Optic Nerve macrophages and Sciatic Nerve macrophages. For each cell type there are 4 replicates, expect for the Brain microglia there are 5 replicates.
Single cell RNA Seq = on steady state Sciatic Nerve macrophages and at different time points (1 day and 5 days) after nerve crush.

Contributor(s)

Ydens E, Amann L, Asselbergh B, Scott CL, Martens L, Sichien D, Mossad O, Blank T, De Prijck S, Low D, Masuda T, Saeys Y, Timmerman V, Stumm R, Ginhoux F, Prinz M, Janssens S, Guilliams M

Citation(s)

Submission date

Feb 03, 2020

Last update date

May 05, 2020

Contact name

Liesbet Martens

E-mail(s)

liesbet.martens@irc.vib-ugent.be

Organization name

VIB-University of Ghent

Department

VIB Inflammation Research Center

Street address

Technologiepark 927

City

Zwijnaarde

ZIP/Postal code

9052

Country

Belgium

Platforms (1)

[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]

Samples (25)

More...

GSM4294022	Steady state scMacs SnMac_StSt_1
GSM4294023	Steady state scMacs SnMac_StSt_2
GSM4294024	Steady state scMacs SnMac_StSt_3

This SubSeries is part of SuperSeries:

Distinct signature, origin and dynamics of macrophages in the peripheral and central nervous system

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BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE144702_RAW.tar	100.6 Mb	(http)(custom)	TAR (of CEL)
GSE144702_RMA_47_samples.txt.gz	8.8 Mb	(ftp)(http)	TXT
Processed data included within Sample table

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