NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE144445 Query DataSets for GSE144445
Status Public on Jan 29, 2023
Title MicroRNA profile of drug resistant metaplastic breast cancer cell lines
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Breast cancer cells and two metaplastic breast cancer cell lines were used: a widely available, HS578T, and a novel line isolated from a metaplastic breast cancer tumor, BAS. Doxorubicin and paclitaxel resistant derivatives of these metaplastic lines were generated and miR profiling performed.
 
Overall design Cells were treated with increasing concentrations of drug until they were able to survive and proliferate after a pulse of cytotoxic drug. HS-TX: HS578T cells, 100 nM paclitaxel, 5 days pulse; HS-DOX: HS-578T cells, 100 nM doxorubicin, 3 days pulse; BAS-TX: BAS cells, 20 nM paclitaxe, 3 days pulse; BAS-DOX, BAS cells, 160 nM doxorubicin, 3 days pulse. For the array experiment, RNA isolated from three independent cultures was pooled and used for each condition.
 
Contributor(s) Yague E, Kumar U
Citation(s) 34298004
Submission date Jan 29, 2020
Last update date Apr 24, 2023
Contact name Ernesto Yague
E-mail(s) ernesto.yague@imperial.ac.uk
Organization name Imperial College London
Department Division of Cancer
Street address Du Cane Road
City London
ZIP/Postal code W12 0NN
Country United Kingdom
 
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (10)
GSM4288400 Primary human mammary epithelial cells
GSM4288401 MCF10A cells
GSM4288402 MCF7 cells - luminal type
Relations
BioProject PRJNA603679

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE144445_RAW.tar 7.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap