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| Status |
Public on Mar 31, 2020 |
| Title |
Investigating chromatin accessibility differences in stem cells and inflammatory cells in heterotopic ossification using ATAC-Seq |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Mice were injured and their response in Mesenchymal Stem Cells (MSC) were studied over a period of seven days. The injury site was either non-immobilized or immobilized. The aim of the project is to study how immobilization affects stemness and inflammatory cell response after injury by investigating chromatin accessibility differences using ATAC-Seq.
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| Overall design |
ATAC-Seq libraries were prepared at the UM Epigenomics Core by using the Omni-ATAC protocol for cells as described in Corces et al., 2017 without modifications.
ATAC-Seq analysis was carried out on all these samples.
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| Contributor(s) |
Padmanabhan K, Huber A, Levi B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 27, 2020 |
| Last update date |
Apr 06, 2020 |
| Contact name |
Bioinformatics Core |
| E-mail(s) |
bioinformatics@umich.edu
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| Organization name |
University of Michigan
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| Street address |
BRCF Bioinformatics Core, 2800 Plymouth Rd
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| City |
Ann Arbor |
| State/province |
Mi |
| ZIP/Postal code |
48109-2800 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (3) |
| GSM4285882 |
D0_MSC: MSC pre-injury at Day 0 |
| GSM4285883 |
D7_MSC_IMMOB: MSC immobilized at Day 7 post-injury |
| GSM4285884 |
D7_MSC_MOB: MSC non-immobilized at Day 7 post-injury |
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| Relations |
| BioProject |
PRJNA603371 |
| SRA |
SRP245427 |
