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Series GSE144168 Query DataSets for GSE144168
Status Public on Jan 28, 2020
Title Retinal endothelial cell phenotypic modifications during experimental autoimmune uveitis: a transcriptomic approach
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Background: Blood-retinal barrier cells are known to exhibit a massive phenotypic change during experimental autoimmune uveitis (EAU) development. In an attempt to investigate the mechanisms of blood-retinal barrier (BRB) breakdown at a global level, we studied the gene regulation of total retinal cells and retinal endothelial cells during non-infectious uveitis. Methods: Retinal endothelial cells were isolated by flow cytometry either in Tie2-GFP mice (CD31+ CD45- GFP+ cells), or in wild type C57BL/6 mice (CD31+ CD45- endoglin+ cells). EAU was induced in C57BL/6 mice by adoptive transfer of IRBP1-20-specific T cells. Total retinal cells and retinal endothelial cells from naïve and EAU mice were sorted and their gene expression compared by RNA-Seq. Protein expression of selected genes was validated by immunofluorescence on retinal wholemounts and cryosections and by flow cytometry. Results: Retinal endothelial cell sorting in wild type C57BL/6 mice was validated by comparative transcriptome analysis with retinal endothelial cells sorted from Tie2-GFP mice, which express GFP under the control of the endothelial-specific receptor tyrosine kinase promoter Tie2. RNA-Seq analysis of total retinal cells mainly brought to light upregulation of genes involved in antigen presentation and T cell activation during EAU. Specific transcriptome analysis of retinal endothelial cells allowed us to identify 82 genes modulated in retinal endothelial cells during EAU development. Protein expression of 5 of those genes (serpina3n, lipocalin 2, ackr1, lrg1 and lamc3) was validated at the level of inner BRB cells. Conclusion: Those data not only confirm the involvement of known pathogenic molecules but further provide a list of new candidate genes and pathways possibly implicated in inner BRB breakdown during non-infectious posterior uveitis.
Overall design Regulation of retinal cell and retinal endothelial cell gene expression during experimental autoimmune uveitis
Contributor(s) Lipski DA, Foucart V, Dewispelaere R, Caspers LE, Defrance M, Bruyns C, Willermain F
Citation(s) 32183784
Submission date Jan 23, 2020
Last update date Mar 24, 2020
Contact name Frederick Libert
Organization name ULB
Department IRIBHM
Lab C2-140
Street address 808 route de Lennik
City Brussels
ZIP/Postal code B-1070
Country Belgium
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (16)
GSM4282376 NR1
GSM4282377 NR2
GSM4282378 NR3
BioProject PRJNA602922
SRA SRP244473

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Supplementary file Size Download File type/resource
GSE144168_Normalized_counts_CPM_Lipski_171130.xlsx 3.9 Mb (ftp)(http) XLSX
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Processed data are available on Series record

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