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| Status |
Public on Apr 15, 2020 |
| Title |
Variation of human neural stem cells generating organizer states in vitro before committing to cortical excitatory or inhibitory neuronal fates [human RNAseq #2] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Better understanding the progression of neural stem cells (NSCs) in the developing cerebral cortex is important for modeling neurogenesis and defining the pathogenesis of neuropsychiat-ric disorders. Here we used RNA-sequencing, cell imaging and lineage tracing of mouse and human in vitro NSCs to model the generation of cortical neuronal fates. We show that con-served signaling mechanisms regulate the acute transition from proliferative NSCs to commit-ted glutamatergic excitatory neurons. As human telencephalic NSCs developed from pluripo-tentcy in vitro, they first transitioned through organizer states that spatially pattern the cortex before generating glutamatergic precursor fates. NSCs derived from multiple human pluripotent lines varied in these early states leading differentially to dorsal or ventral telencephalic fates. This work furthers systematic analysis of the earliest patterning events that generate the major neuronal trajectories of the human telencephalon. The gene amplitudes for the GWCoGAPS patterns in this data can be found in the supplemental files.
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| Overall design |
Of the 3 series in this super series, this covers RNAseq of 2 replicate iPSC lines from each of 3 new donors during 8, 17, 30 and 32 days of neural differentiation (used in Figure 5)
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| Contributor(s) |
Micali N, Kim SK, Diaz-Bustamante M, Stein-O'Brien G, Seo S, Shin JH, Rash BG, Ma S, Wang Y, Olivares NA, Arellano J, Maynard KR, Fertig E, Cross AJ, Burli R, Brandon NJ, Weinberger DR, Chenoweth JG, Hoeppner DJ, Sestan N, Rakic P, Colantuoni C, McKay RD |
| Citation(s) |
32375049 |
| Submission date |
Jan 23, 2020 |
| Last update date |
Jul 15, 2020 |
| Contact name |
Carlo Colantuoni |
| E-mail(s) |
ccolantu@jhmi.edu
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| Phone |
4104931439
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| Organization name |
Johns Hopkins Univ. School of Medicine
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| Department |
Neurology
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| Street address |
733 N Broadway
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE144159 |
Variation of human neural stem cells generating organizer states in vitro before committing to cortical excitatory or inhibitory neuronal fates |
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| Relations |
| BioProject |
PRJNA602912 |
| SRA |
SRP244392 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE144157_RAW.tar |
2.3 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE144157_RPKMtable_BRNiPSCnpcNRN.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| GSE144157_iPSC6lines_BRNiPSCnpcNRN_GWCoGAPS_p30.xlsx |
7.0 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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