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Series GSE143742 Query DataSets for GSE143742
Status Public on Dec 15, 2020
Title Senescence and TGF-β producing Tregs drive HIV persistence and impaired immune homeostasis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The purpose of this study was to determine differences in gene expression profiles between Immune Responders (IR) and Immune non-Responders (INR) individuals. We have observed significant changes in the gene expression profiles between IRs and INRs and identified a subgroup of INR subjects that has a unique/distinct transcriptional profile and that clustered the furthest from IR subjects.
 
Overall design Immune responder and non-responder individuals included in the study were HIV-infected and ART-treated for at least 3 years. Immune non-responder patients had CD4 T cells <350/µL and immune responder patients had CD4 T cells >500/µL. Whole blood was collected and lysed in RLT for RNA extraction. Reverse transcription reactions were performed to obtain cDNAs which were hybridized to the Illumina Human HT-12 version 4 Expression BeadChip according to the manufacturer’s instruction, and quantified using an Illumina iScan System. The data were collected with Illumina GenomeStudio software.
 
Contributor(s) Ghneim K, Arunkumar Sharma A, Periera Ribeiro S, Arumagam S, Darko S, Fourati S, Ahlers J, Kulpa D, Xu X, Blasco Lopes F, Brehm J, Talla A, Matassoli F, Rodriguez B, Shive C, Cristescu R, Loboda A, Balderas R, Wang I, Hunt P, Lamarre D, Douek D, Hazuda D, Lederman MM, Deeks S, Sekaly R
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Submission date Jan 15, 2020
Last update date Dec 18, 2020
Contact name Rafick Pierre-Sekaly
E-mail(s) rafick.sekaly@case.edu, rafick.sekaly@emory.edu
Organization name Case Western Reserve University
Department Pathology
Street address 2103 Cornell Avenue
City Cleveland
State/province OH
ZIP/Postal code 44106
Country USA
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (61)
GSM4273542 6235783009_J: Immune Responder (IR)
GSM4273543 6260971076_A: Immune non-Responder (INR)
GSM4273544 6260971076_B: Immune non-Responder (INR)
Relations
BioProject PRJNA601471

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE143742_RAW.tar 26.2 Mb (http)(custom) TAR
GSE143742_non-normalized.txt.gz 3.3 Mb (ftp)(http) TXT
GSE143742_normalized.txt.gz 8.9 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record
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