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Series GSE143702 Query DataSets for GSE143702
Status Public on Jul 10, 2020
Title Integrative analysis of transcriptome and proteome sheds light on functional differences between human salivary glands
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Saliva aids in the predigestion of food and perception of taste. It helps to maintain the integrity of the mineralized tooth and epithelial surfaces in the mouth, and shields the oro-digestive tract from environmental hazards and invading pathogens. Although salivary glands and saliva fluid are biologically and functionally inseparable, they have thus far been investigated as separate entities. To bridge this gap, we performed an integrative analysis of the transcriptome of 27 samples collected from the major human adult and fetal major salivary glands - submandibular, sublingual, and parotid - along with mass-spectrometry-based saliva proteome data and immunohistochemical localization in glandular tissue. Our results suggest that functional maturation at the transcriptome level occurs late in gland development, and is driven mainly by the transcription of genes that code for secreted saliva proteins. We further provide evidence that protein dosage of the most abundant salivary proteins secreted by the salivary glands is predominantly regulated at the transcriptome level. Finally, we demonstrate distinct transcriptomic profiles of each major salivary gland type that reveal functional specialization and will aid in future clinical analyses. Our study provides the hitherto most comprehensive RNAseq dataset of healthy salivary glands in humans, thus establishing a robust framework for deeper studies of saliva and salivary gland biology, development, and evolution, ultimately paving the way for better understanding the importance of these craniofacial secretory organs in health and their malfunctions in disease.
 
Overall design We report an integrative analysis of the transcriptome of 27 samples collected from the human adult and fetal major salivary glands - submandibular, sublingual, and parotid
 
Contributor(s) Saitou M, Xu E, Knox SM, Gokcumen O
Citation(s) 33207190
Submission date Jan 15, 2020
Last update date Nov 29, 2020
Contact name Marie Saitou
Organization name NMBU
Street address ├ůs
City Viken
State/province Viken
ZIP/Postal code 1430
Country Norway
 
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (27)
GSM4272857 Adult_Sublingual_A.430_S15
GSM4272858 Adult_Parotid_A.437_S7
GSM4272859 Adult_Parotid_A.439_S8
Relations
BioProject PRJNA601418
SRA SRP242038

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Supplementary file Size Download File type/resource
GSE143702_saliva_master_2019.7.xlsx 11.0 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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