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| Status |
Public on Aug 06, 2020 |
| Title |
Targets of transduced Foxa and Hnf4alpha proteins in mouse embryonic fibroblasts (MEFs). |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We have found three specific combinations of two transcription factors, comprising Hnf4alpha plus Foxa1, Foxa2 or Foxa3, could convert mouse embryonic fibroblasts (MEFs) into cells that closely resemble hepatocytes in vitro. Then we used ChIP-seq to explore the targets of the transduced Foxa and Hnf4alpha proteins in MEFs.
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| |
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| Overall design |
Using anti-Foxa antibodies and an anti-Hnf4alpha antibody we performed ChIP-Seq for MEFs.
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| Contributor(s) |
Suzuki A, Ohkawa Y, Nagasaki M, Horisawa K, Ueno K |
| Citation(s) |
32755593 |
| |
| Submission date |
Jan 10, 2020 |
| Last update date |
Aug 10, 2020 |
| Contact name |
Atsushi Suzuki |
| Organization name |
Medical Institute of Bioregulation, Kyushu University
|
| Department |
Division of Organogenesis and Regeneration
|
| Street address |
3-1-1 Maidashi, Higashi-ku
|
| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA600509 |
| SRA |
SRP241210 |
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