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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 08, 2020 |
| Title |
ISX-9 manipulates endocrine progenitor fate revealing conserved intestinal lineages in mouse and human |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Objective: Enteroendocrine cells (EECs) survey the gut luminal environment and co-ordinate hormonal, immune and neuronal responses to it. They exhibit well characterized physiological roles ranging from the control of local gut function to whole body metabolism, but little is known regarding the regulatory networks controlling their differentiation, especially in human gut. The small molecule Isoxazole-9 (ISX-9) has been shown to stimulate neuronal and pancreatic beta-cell differentiation, both closely related to EEC differentiation. Our aim was to use ISX-9 as a tool to explore EEC differentiation. Methods: We investigated the effects of ISX-9 on EEC differentiation in mouse and human intestinal organoids, using real time quantitative PCR, fluorescent activated cell sorting, immunostaining and single cell RNA sequencing. Results: ISX-9 increased the number of neurogenin3 (Ngn3) positive endocrine progenitor cells and upregulated NeuroD1 and Pax4, transcription factors which play roles in mouse EEC specification. Single cell analysis revealed induction of Pax4 expression in a developmentally late Ngn3+ population of cells and potentiation of genes associated with progenitors biased towards serotonin-producing enterochromaffin (EC) cells. This coincided with enrichment of organoids with functional EC cells which was partly dependent on stimulation of calcium signalling in a population of cells residing outside the crypt base. Inducible Pax4 overexpression, in ileal organoids, uncovered its importance as a component of early human endocrine specification and highlighted the potential existence of two major endocrine lineages, the early appearing enterochromaffin lineage and the later developing peptidergic lineage which contains classical gut hormone cell types. Conclusion: Our data provide proof-of-concept for the controlled manipulation of specific endocrine lineages with small molecules, whilst also shedding new light on human EEC differentiation and its similarity to mouse. Given their diverse roles, understanding endocrine lineage plasticity and its control could have multiple therapeutic implications.
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| Overall design |
Single-cell RNA-seq data from mouse Ngn3-RFP small intestine organoids (Control and ISX-9 treated)
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| Contributor(s) |
Tsakmaki A, Fonseca Pedro P, Pavlidis P, Hayee B, Bewick GA |
| Citation(s) |
32180555 |
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| Submission date |
Jan 07, 2020 |
| Last update date |
Mar 23, 2020 |
| Contact name |
Patricia Fonseca Pedro |
| E-mail(s) |
patricia.pedro@kcl.ac.uk
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| Organization name |
King's College London
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| Street address |
Hodgkin Building, 2nd Floor, Newcomen Street
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| City |
London |
| State/province |
I am not in the U.S. or Canada |
| ZIP/Postal code |
SE1 1UL |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
| GSM4253870 |
Organoid Ngn3-RFP positive single cell transcriptomes, DMSO treated |
| GSM4253871 |
Organoid Ngn3-RFP positive single cell transcriptomes, ISX-9 treated |
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| Relations |
| BioProject |
PRJNA599344 |
| SRA |
SRP239774 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE143221_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR (of TSV) |
| GSE143221_readme_demultiplexing_Cel-seq2_data.pdf |
811.8 Kb |
(ftp)(http) |
PDF |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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