 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 03, 2020 |
| Title |
Identification and specification of the mouse skeletal stem cell. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
How are skeletal tissues derived from skeletal stem cells? Here, we map bone, cartilage, and stromal development from a population of highly pure, postnatal skeletal stem cells (mouse skeletal stem cells, mSSCs) to their downstream progenitors of bone, cartilage, and stromal tissue. We then investigated the transcriptome of the stem/progenitor cells for unique gene-expression patterns that would indicate potential regulators of mSSC lineage commitment. We demonstrate that mSSC niche factors can be potent inducers of osteogenesis, and several specific combinations of recombinant mSSC niche factors can activate mSSC genetic programs in situ, even in nonskeletal tissues, resulting in de novo formation of cartilage or bone and bone marrow stroma. Inducing mSSC formation with soluble factors and subsequently regulating the mSSC niche to specify its differentiation toward bone, cartilage, or stromal cells could represent a paradigm shift in the therapeutic regeneration of skeletal tissues.
|
| |
|
| Overall design |
Single-cell RNA-sequencing (C1 platform) of FACS-sorted mouse skeletal stem, progenitor, and stromal populations (mSSC, CSP, Thy+, and 6C3+).
|
| |
|
| Contributor(s) |
Chan CF, Seo EY, Sinha R, Weissman IL, Longaker MT |
| Citation(s) |
25594184 |
| |
| Submission date |
Jan 02, 2020 |
| Last update date |
Apr 03, 2020 |
| Contact name |
Charles Chan KF |
| E-mail(s) |
chazchan@gmail.com
|
| Organization name |
Stanford
|
| Street address |
265 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5461 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (227)
|
|
| This SubSeries is part of SuperSeries: |
|
| Relations |
| BioProject |
PRJNA598920 |
| SRA |
SRP239468 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142873_Skeletal_scRNAseq_FPKM_matrix.txt.gz |
2.4 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...