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Series GSE142873 Query DataSets for GSE142873
Status Public on Jan 03, 2020
Title Identification and specification of the mouse skeletal stem cell.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary How are skeletal tissues derived from skeletal stem cells? Here, we map bone, cartilage, and stromal development from a population of highly pure, postnatal skeletal stem cells (mouse skeletal stem cells, mSSCs) to their downstream progenitors of bone, cartilage, and stromal tissue. We then investigated the transcriptome of the stem/progenitor cells for unique gene-expression patterns that would indicate potential regulators of mSSC lineage commitment. We demonstrate that mSSC niche factors can be potent inducers of osteogenesis, and several specific combinations of recombinant mSSC niche factors can activate mSSC genetic programs in situ, even in nonskeletal tissues, resulting in de novo formation of cartilage or bone and bone marrow stroma. Inducing mSSC formation with soluble factors and subsequently regulating the mSSC niche to specify its differentiation toward bone, cartilage, or stromal cells could represent a paradigm shift in the therapeutic regeneration of skeletal tissues.
Overall design Single-cell RNA-sequencing (C1 platform) of FACS-sorted mouse skeletal stem, progenitor, and stromal populations (mSSC, CSP, Thy+, and 6C3+). 
Contributor(s) Chan CF, Seo EY, Sinha R, Weissman IL, Longaker MT
Citation(s) 25594184
Submission date Jan 02, 2020
Last update date Apr 03, 2020
Contact name Charles Chan KF
Organization name Stanford
Street address 265 Campus Drive
City Stanford
State/province CA
ZIP/Postal code 94305-5461
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (227)
GSM4246876 6C3-N701-S503_S109
GSM4246877 6C3-N701-S505_S121
GSM4246878 6C3-N701-S506_S133
This SubSeries is part of SuperSeries:
GSE64447 Neonatal Skeletal Progenitors
BioProject PRJNA598920
SRA SRP239468

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE142873_Skeletal_scRNAseq_FPKM_matrix.txt.gz 2.4 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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