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| Status |
Public on Jun 08, 2020 |
| Title |
Single cell RNA-sequencing of microglia in 3XTg-AD mice treated with anti-Nk1.1 depleting antibodies or isotype control |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This project aims to understand the effects of NK cell depletion in the single-cell gene expression of microglia in mice with Alzheimer's disease pathology. We used 10Xgenomics technique to examine the single-cell transcriptomes of microglia in mice treated with anti-NK1.1 depleting antibodies and isotype controls.
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| Overall design |
We sorted microglia from 3XTg-AD mice treated with anti-Nk1.1 depleting antibodies or isotype controls. Single-cell RNA-seq was performed using 10X Genomics Chromium isingle-cell controller and 5' gene expression kit.
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| Contributor(s) |
Yang Q, Zhang Y |
| Citation(s) |
32503894 |
| |
| Submission date |
Jan 02, 2020 |
| Last update date |
Jun 08, 2020 |
| Contact name |
Qi Yang |
| E-mail(s) |
qy117@rutgers.edu
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| Phone |
6102339301
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| Organization name |
Rutgers
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| Lab |
Yang
|
| Street address |
89 French St
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| City |
New Brunswick, NJ 089 |
| State/province |
New Jersey |
| ZIP/Postal code |
08901 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA598680 |
| SRA |
SRP239316 |
