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Series GSE142825 Query DataSets for GSE142825
Status Public on Oct 08, 2020
Title EGFR/Src/Erk-Stabilized YTHDF2 Promotes Cholesterol Dysregulation and Invasive Growth of Glioblastoma [RNA-Seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Epidermal growth factor receptor (EGFR) signaling is constitutively activated in majority of GBM and is associated with a worse prognosis. Here we show that EGFR is responsible for overexpression of the m6A "reader" YTHDF2 in GBM through the EGFR/Src/ERK signaling pathway. YTHDF2 overexpression clinically correlates with poor glioma patient prognosis. EGFR signaling stabilizes YTHDF2 protein through phosphorylation of YTHDF2 serine 39 and threonine 381 by ERK1/2. YTHDF2 is required for GBM cell proliferation, invasion and tumorigenesis. YTHDF2 facilitates m6A-dependent mRNA decay of LXRA and HIVEP2, both are genes impacting glioma patient survival. YTHDF2 promotes tumorigenesis of GBM cells largely through downregulation of LXRA and HIVEP2. Further, YTHDF2 inhibits LXRA-dependent cholesterol homeostasis in GBM cells. Together, our findings extend the landscape of EGFR downstream circuit, uncover novel function for YTHDF2 in GBM tumorigenesis, and highlight an essential role of RNA m6A methylation in cholesterol homeostasis.
Overall design Expression profiles were generated from GSC11 human glioblastoma stem cell lines transfected with control or YTHDF2 siRNA, which were collected to extract total RNA, and mRNA sequencing was performed.
Contributor(s) Huang S
Citation(s) 33420027
Submission date Jan 02, 2020
Last update date Jan 26, 2021
Contact name Youqiong Ye
Phone +8613661565591
Organization name Shanghai Jiao Tong University School of Medicine
Street address 280 Chongqing south road
City Shanghai
ZIP/Postal code 200025
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM4751140 siControl-1
GSM4751141 siControl-2
GSM4751142 siYTHDF2-1
This SubSeries is part of SuperSeries:
GSE142828 EGFR/Src/Erk-Stabilized YTHDF2 Promotes Cholesterol Dysregulation and Invasive Growth of Glioblastoma
BioProject PRJNA598564
SRA SRP239270

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Supplementary file Size Download File type/resource
GSE142825_DifferentialExpression.xlsx 4.2 Mb (ftp)(http) XLSX
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