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Series GSE142786 Query DataSets for GSE142786
Status Public on Nov 01, 2021
Title Single-cell RNA-seq analysis reveals reduction of B cells in the bone marrow promote fracture healing
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Recent studies have identified B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation. However, it remains unknown how B cells participate in the healing of fractures. Here we find that B cells decrease significantly during fracture healing and returned to normal levels after that by single-cell profiling. Furthermore, we found that exosomes derived from B cells inhibited the differentiation of osteoblasts and osteoclasts in vitro. Additionally, injection of these exosomes in mice delayed fracture healing. Thus, maintaining low B cell levels in the bone marrow microenvironment promotes fracture healing.
Overall design scRNA profiles of fracture at different stage (control group, 30th day)
Contributor(s) Wang R, Tang H
Citation(s) 34819916
Submission date Dec 31, 2019
Last update date Dec 07, 2021
Contact name Renkai Wang
Organization name changhai hospital
Street address 168 Changhai Road,
City shanghai
ZIP/Postal code 200433
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (2)
GSM4240163 control group_scRNA-seq
GSM4240164 30th Day_scRNA-seq
BioProject PRJNA598334
SRA SRP239180

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Supplementary file Size Download File type/resource
GSE142786_AllSample.counts.tsv.gz 15.3 Mb (ftp)(http) TSV
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