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Series GSE142784 Query DataSets for GSE142784
Status Public on Jun 06, 2020
Title Dissecting the transcriptomic landscape of the human intrahepatic cholangiocarcinoma by single cell RNA-sequencing analysis.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Intrahepatic cholangiocarcinoma (ICC) is the second most common malignancy arising in the liver, featured with high tumor heterogeneity and dismal prognosis. The cellular disversity and molecular basis of tumor cells interacted with surrounding niche cells remains poorly understood, limiting the development of targeted anti-tumor therapies.
To investigate the molecular diversity of fibroblast population, 2 tumor samples: ICC32S and STROMA24T were recruited in our study to perform fibroblast enriched scRNA-seq
Overall design To comprehensively elucidate the ICC heterogeneity, we employed single-cell RNA sequencing (scRNA-seq) to profile 31,302 cells of five ICC samples, as well as three paired adjacent non-tumor tissues. Our results identify 31 cell subtypes and the heterogeneous malignant subtype was highly patient-specific, which has differential proliferative, migratory and inflammation phenotype.
Contributor(s) Zhang M, Yan X, Liu B
Citation Zhang M, Yang H, Wan L, Wang Z, Wang H, Ge C, Liu Y, Hao Y, Zhang D, Shi G, Gong Y, Ni Y, Wang C, Zhang Y, Xi J, Wang S, Shi L, Zhang L, Yue W, Pei X, Liu B, and Yan X. Single cell transcriptomic architecture and intercellular crosstalk of human intrahepatic cholangiocarcinoma. Journal of Hepatology (2020). doi:10.1016/j.jhep.2020.05.039
Submission date Dec 31, 2019
Last update date Jun 06, 2020
Contact name Min Zhang
Phone 13241302826
Organization name Academy of Military Medical Sciences
Department The Fifth Medical Center of Chinese PLA General Hospital
Street address No. 8 East Road, Fengtai District
City Beijing
State/province Beijing
ZIP/Postal code 100071
Country China
Platforms (2)
GPL20795 HiSeq X Ten (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (2)
GSM4240155 STROMA24T
GSM4240156 CC32S
BioProject PRJNA598336
SRA SRP239174

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Supplementary file Size Download File type/resource
GSE142784_RAW.tar 25.1 Mb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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