NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE142708 Query DataSets for GSE142708
Status Public on Apr 09, 2020
Title Transcriptional response to IFN-κ and IFN-κDual in A549 cell line
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Type I interferon (IFN) is the first line of defense against virus infection. By using both in vivo and in vitro influenza infection models, we found that type I IFN-κ, limited the replication of influenza viruses by stimulating a IFNAR-MAPK-cFos-CHD6 axis. Similarly, Zika virus (ZIKV) was also highly sensitive to IFN-κ-mediated suppression. With an IAV infected mouse model, we found that IFN-κ was the earliest responding type I interferon among all known members in mice after H9N2 infection, a low-pathogenic Avian Influenza, whereas this early induction did not occur upon highly pathogenic H7N9 infection. IFN-κ can efficiently contain both low- and high-pathogenic influenza replication in cultured human lung cells, and CHD6 was the major effector responsive molecule for IFN-κ, but not for IFN-α/β. Furthermore, we discovered that both IFNAR1 and IFNAR2 subunits of type I interferon receptor and their downstream axis of p38-cFos are engaged in IFN-κ signaling cascade to acti vate CHD6, which didn`t require STAT1 activity. In addition, we showed that the pre-treatment with IFN-κ before IAV challenge protected mice from high mortality. Altogether, our study identified an IFN-κ-specific pathway that suppressed influenza A virus in vitro and in vivo. Thus, IFN-κ may have potential as a new prevention and treatment agents against emerging viruses
 
Overall design RNAseq of A549 cells transfected with plasmids encoding WT IFN-κ, IFN-κ Dual, or pSV1.0 empty vector
RNA was isolated 24 hours after transfected. Samples were collected in biological triplicate and pSV1.0 empty vector was control.
 
Contributor(s) He Y
Citation(s) 32265337, 36034707
Submission date Dec 29, 2019
Last update date Aug 31, 2022
Contact name Yongquan He
E-mail(s) yongquanhe2012@163.com
Organization name Fudan University
Street address No.2901 Caolang Road
City Shanghai
ZIP/Postal code 201508
Country China
 
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (9)
GSM4238108 A549 cells, IFN-κ_1
GSM4238109 A549 cells, IFN-κ_2
GSM4238110 A549 cells, IFN-κ_3
This SubSeries is part of SuperSeries:
GSE142709 IFN-kappa Suppresses the Replication of Influenza A Virus through IFNAR-MAPK-Fos-CHD6 Axis
Relations
BioProject PRJNA598133

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE142708_RAW.tar 110.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap