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Series GSE142662 Query DataSets for GSE142662
Status Public on Apr 02, 2021
Title Transcriptomic analysis by RNA-seq unveils distinct gene expression profile of U397 cells depleted of NSPs
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Characterization of transcriptomic changes associated with genetic ablation of three neutrophil serine proteases (CTSG, ELANE, PRTN3). U937 cells depleted of CTSG, ELANE, and PRTN3 simultaneously (ΔNSPs) were generated using the CRISPR-Cas9 system and sgRNAs. RNA-Seq analysis revealed transcriptomic changes between control cells and ΔNSPs, with 170 and 220 genes up- or down-regulated respectively.
Overall design RNA-Seq analysis of three control lines of U937 cells and three independent clones of ΔNSPs U937 cells
Contributor(s) Cheung P, Schaffert S, Chang SE, Mellins ED, Khatri P, Utz PJ, Kuo AJ
Citation(s) 34017121
Submission date Dec 27, 2019
Last update date May 23, 2021
Contact name Purvesh Khatri
Organization name Stanford University
Department BMIR
Street address 279 Campus Dr. Beckman Center B235A
City Stanford
State/province California
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM4237403 U937 cells, Control_1 (RNA-seq)
GSM4237404 U937 cells, Control_2 (RNA-seq)
GSM4237405 U937 cells, Control_3 (RNA-seq)
BioProject PRJNA597899
SRA SRP238918

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Supplementary file Size Download File type/resource
GSE142662_counts_raw.csv.gz 236.0 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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