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Series GSE142637 Query DataSets for GSE142637
Status Public on May 15, 2020
Title Interferon lambda promotes immune dysregulation and tissue inflammation in TLR7-induced lupus [scRNA-seq]
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Interferon lambdas share important functional similarities with type I interferons, but their role in inflammation and autoimmune disease remains controversial and is not well studied. Here, we present the first evidence that interferon lambda is pathogenic and has nonredundant functions in TLR7-associated lupus inflammation. Most notably, we found that interferon lambda promotes systemic immune dysregulation through localized effects in the skin and kidneys. These data identify a novel role for interferon lambda in lupus immunobiology and tissue-specific pathology.
Overall design This dataset includes 6 scRNA samples. Immune cells were isolated from mouse spleen and human peripheral blood. Cells were treated with recombinant interferon alpha or interferon lambda for 4 hours. Single cell RNA seq analysis was subsequently performed to analyze responsiveness to stimulation with interferon alpha and interferon lambda cytokine across different immune cell clusters.
Contributor(s) Goel RR, Wang X, O'Neil LJ, Nakabo S, Hasneen K, Gupta S, Wigerblad G, Blanco LP, Kopp JB, Morasso MI, Kotenko SV, Yu Z, Carmona-Rivera C, Kaplan MJ
Citation(s) 32094169
Submission date Dec 26, 2019
Last update date May 15, 2020
Contact name Hong-wei Sun
Organization name NIAMS
Department Office of Science & Technology
Lab Biodata Mining & Discovery Section
Street address 9000 Rockville Pile
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (2)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (6)
GSM4233278 scRNA Human Blood IFN alpha
GSM4233279 scRNA Human Blood IFN lambda
GSM4233280 scRNA Human Blood Untreated
BioProject PRJNA597786
SRA SRP238856

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Supplementary file Size Download File type/resource
GSE142637_RAW.tar 214.5 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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