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Series GSE142631 Query DataSets for GSE142631
Status Public on Apr 01, 2020
Title Transcriptomic programming of human CD8+ T cells. By pDCs and cDC2s jin the context of DC-based cancer vaccines
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Cancer vaccines utilizing naturally circulating dendritic cell (DC) subsets, such as plasmacutoid DCs (pDCs) and type 2 convential DCs (cDC2s), have demonstrated their potential as a therapy. For melanoma in recent clinical trials. These DC vaccines aim to. human. DC subsets on the. T cell transcriptional program, which. forms the. molecular. basis. of an. antitumor. T cell response, is. poorly understood. In. this study, we investigated the eraly gene expressionsignature of CD8+ T. cells following. stimulation by pDCs or cDC2s. in. a culture system that mimics. the. current protocol of primary DC-based cancer vaccines. Our results demonstrate that pDCs and cDC2s induce a remarkably similar transcriptomic profile in CD8_ T cells, which. is tailored for increased effector function, survival and sensitivity. towards secondary signals. Nonetheless, differences between pDC- and cDC2-induced T cell gene expression signatures encompass genes with a role in proliferation, cytolytic capacity and differentiation. Combining both DC subsets results in a T cell. transcriptomic program that. is very. similar to. the program induced by. pDCs alone. The results suggest that the. choice of DC subsets for use in cancer vaccines impacts the induced antitumor CD8+ T cell response.
Overall design Cd8 Tcells and DC subtypes were isolated from blood from 3 donors
Antigen: NY-ESO-1 peptide (SLLMWITQV)
Contributor(s) Textor J
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Submission date Dec 26, 2019
Last update date Apr 01, 2020
Contact name Jessie van Buggenum
Organization name Radboud University
Street address Heyendaalseweg 135
City Nijmegen
ZIP/Postal code 6525 AJ
Country Netherlands
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (18)
GSM4233194 Donor-2 CD8 Tcell coculture with CD1c+ cells with antigen
GSM4233195 Donor-3 CD8 Tcell coculture with CD1c+ cells with antigen
GSM4233196 Donor-4 CD8 Tcell coculture with CD1c+ cells with antigen
BioProject PRJNA597777
SRA SRP238849

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Supplementary file Size Download File type/resource
GSE142631_countdata.txt.gz 739.6 Kb (ftp)(http) TXT
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