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Series GSE142607 Query DataSets for GSE142607
Status Public on Dec 09, 2020
Title Metabolism in synovial macrophages are reprogrammed by synovial fibroblasts under inflammatory condition
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Macrophages have plasticity to adapt microenvironment. In joint tissue, synovial macrophages (SM) and synovial fibroblasts (SF) are maintained in the homeostasis. In Rheumatoid arthritis, crosstalk between SM and SF via inflammatory response induce abnormal activation in respective cells and contribute to disease progression. However, the activation mechanisms in SM which are encouraged by SF are largely unclear. Here, we demonstrated metabolic reprogramming and immunological activation in SM by secretary stimulations from SF using primary culture synovial cell derived from arthritis model mice. To analyze interaction between SM and SF, primary culture of murine synovial cells was performed, respectively. RNA-seq analysis showed SF express abundant secretion-related gene. Thus, we investigated whether conditioned medium from SF (SF-CM) affects biological activity in SM. As the results, SF-CM condition induced both glycolysis and mitochondrial respiration to SM with increased uptake of glucose and glutamine at least, accompanied with cell survival. In addition, several inflammation markers were also upregulated in SM by SF-CM condition. Taken together, these results suggest that metabolic reprogramming were induced in SM by secretory stimulations from SF, followed by activated inflammatory response and long-live. These indicate that such phenotypes of SM may contribute to chronic inflammation in Rheumatoid arthritis
Overall design mRNA profiles of primary synovial macrophages and synovial fibroblasts obtained from swollen ankle in CAIA mice were generated by deep sequencing, in triplicate, using Illumina Miseq.
Contributor(s) Imai Y, Saeki N
Citation(s) 33256735
Submission date Dec 26, 2019
Last update date Dec 09, 2020
Contact name Yuuki Imai
Phone +81-89-960-5925
Organization name Ehime University
Department Proteo-Science Center
Lab Division of Integrative Pathophysiology
Street address Shitsukawa
City Toon
State/province Ehime
ZIP/Postal code 791-0295
Country Japan
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (9)
GSM4232802 CAIA mice, SM rep1
GSM4232803 CAIA mice, SM rep2
GSM4232804 CAIA mice, SM rep3
BioProject PRJNA597752
SRA SRP238820

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE142607_FPKM_SM_SF.xlsx 2.9 Mb (ftp)(http) XLSX
GSE142607_Processed_data_modified.xlsx 7.3 Mb (ftp)(http) XLSX
GSE142607_tmm_SM_SF.xlsx 6.0 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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