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Series GSE142567 Query DataSets for GSE142567
Status Public on Dec 01, 2021
Title RNA sequencing (RNA-SEQ) of FOXM1 knockdown by siRNA in ovarian cancer cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose:Forkhead box M1 (FOXM1) transcription factor is ubiquitously expressed in embryonic tissues but rare in differentiated cells. Increased expression of FOXM1 is observed in a variety of human malignancies. Here, we sought to characterize the expression and tumorigenic roles of FOXM1 in high-grade serous ovarian carcinoma (HGSOC). Experimental Design:TCGA dataset were analyed to identify potential master transcriptional regulators in HGSOC. Immunohistochemistry was performed to evaluate the clinical significance of FOXM1 in HGSOC. Invasion, clonogenic assays were conducted to determine to functional role of FOXM1. ChIP-seq and luciferase analyses were utilized to identify FOXM1 direct target genes in HGSOC. Association between mutant p53 and FOXM1 expression was investigated through TCGA data analysis, immunohistochemistry stainingand western blot. Results: We identified FOXM1 as a potential key transcription factor in ovarian cancer. FOXM1 was markedly increased in HGSOCs and high FOXM1 expression correlated with poor prognosis in HGSOC patients. Mechanistically, FOXM1 regulated CCNF and KIF20A at transcriptional level through binding on their promoters. Consistently, CCNF and KIF20A were elevated in HGSOCs and correlated with poor prognosis. Importantly, mutant p53 contribute to the high expression of FOXM1 in HGSOCs. Conclusions: These data reveal FOXM1 as a driver oncogenic transcription factor that promotes ovarian cancer malignancy which might be a potential drug target in HGSOC.
Overall design The assay was performed on three biological triplicates as well as three experimental triplictes of FOXM1 knockdown and control cells.
Contributor(s) Li Y, Liu Z, Kong B
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Submission date Dec 24, 2019
Last update date Dec 01, 2021
Contact name David Li
Organization name Shandong University
Street address 44 Wenhua Xi Road,Jinan
City jinan
ZIP/Postal code 250012
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM4232015 si-NC#1
GSM4232016 si-NC#2
GSM4232017 si-NC#3
BioProject PRJNA597472
SRA SRP238685

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Supplementary file Size Download File type/resource
GSE142567_B2780si-Foxm1_case_vs_B2780si-NC_ctrl_all_list.xls.gz 2.2 Mb (ftp)(http) XLS
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Raw data are available in SRA
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