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Series GSE142556 Query DataSets for GSE142556
Status Public on Oct 21, 2020
Title The oncogenomic function of androgen receptor in esophageal squamous cell carcinoma is directed by GATA3
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Esophageal squamous cell carcinoma (ESCC) is the sixth leading cause of cancer death worldwide. Emerging evidence suggests that the androgen receptor (AR) is involved in ESCC tumorigenesis. However, how AR exerts its genomic functions in ESCC remains unknown. Here, by defining AR cistromes and analyzing androgen-regulated transcriptomes, we find that AR downregulates the majority of its target genes in ESCC cells. We further find that the pioneer factor GATA3 governs AR-repressed transcription by recruiting SMRT/HDAC3 co-repressors to target gene loci. Importantly, genetic inhibition of GATA3 or pharmacological inhibition of AR/HDAC3 relieves AR-mediated gene repression, leading to ESCC cell growth inhibition in vitro and in vivo. Our findings reveal molecular mechanisms underlying the oncogenomic function of AR in ESCC and identify the GATA3-directed AR transcriptional repression program as a therapeutic target for ESCC.
Overall design AR cistrome and mRNA profiles of ESCC cells treated with vehicle or R1881 were generated by deep sequencing using Illumina HiSeq4000
Contributor(s) Chen Z, Huang F, Wang Q
Citation(s) 33139924
Submission date Dec 23, 2019
Last update date Jan 20, 2021
Contact name Zhong Chen
Organization name Duke University
Department Pathology
Lab Room 1027B, GSRB1
Street address 905 S. LaSalle Street
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
Platforms (2)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (30)
GSM4231884 AR_ChIP_vehicle, rep1
GSM4231885 AR_ChIP_vehicle, rep2
GSM4231886 AR_ChIP_vehicle, rep3
BioProject PRJNA597367
SRA SRP238627

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE142556_410_N20_r1881.ucsc.bedGraph.gz 16.7 Mb (ftp)(http) BEDGRAPH
GSE142556_410_N20_v.ucsc.bedGraph.gz 20.5 Mb (ftp)(http) BEDGRAPH 440.8 Mb (ftp)(http) BW 389.4 Mb (ftp)(http) BW
GSE142556_RAW.tar 810.0 Kb (http)(custom) TAR (of TXT) 438.0 Mb (ftp)(http) BW 396.4 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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