GEO help: Mouse over screen elements for information.
|Public on Dec 22, 2022
|Effects of deproteinized extract of calf blood on the energy metabolism of acute hypoxia model mice and its targets
|Expression profiling by array
|Deproteinized extract of calf blood (DECB) can improve the transport and utilization of blood oxygen and glucose in hypoxic tissue cells, and accelerate the oxidative phosphorylation process of mitochondria, to improve the energy metabolism of cells. Due to its complex composition, the mechanism of DECB on energy metabolism is not entirely clear yet. Effects of DECB on the energy metabolism of acute hypoxia model mice were observed by detecting glucose, lactic acid, and protein and ATP contents in the brain, heart and liver of the mice. Differentially expressed genes related to the effect of DECB on the energy metabolism of acute hypoxia model mice were screened with the mRNA chip and the correlation analysis was performed. Hmgcs2, Cpt1a, Angptl4, Cyp8b1 and Ehhadh genes were involved in the energy metabolism in mice, and mainly closely related to some biological processes, including ABC transporters, adipocytokine signaling pathway and PPAR signaling pathway. DECB can improve the body energy metabolism in mice, of which Angptl4, Cyp8b1 and Ehhadh are important factors in regulating pathways of glucose metabolism, Hmgcs2 gene can directly provide the energy in the lack of energy in the body, and Cpt1a may regulate the inflammatory reaction and promote the energy metabolism.
|Male ICR mice, weighing 18-22g, were randomly divided into two groups, namely control group (CON) and DECB group, and 12 mice in each group. All the mice were administered continuously for 5 weeks and their body weights were weighed once every three days. Mice in the control group and DECB group were placed in containers with the same volume, the container mouth was evenly coated with a layer of vaseline, and all the container covers were closed at the same time while the timing started to ensure the start time of closing was the same. The asthmatic breathing accompanied by a slight convulsion of mice was taken as the indicator to immediately open the closed container, and then the hypoxia tolerance time of mice was observed and recorded.
|Dec 23, 2019
|Last update date
|Dec 24, 2022
|College of Pharmacy, Beihua University
|Binjiang Road 3999,jilin city,132013
|Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
|TAR (of TXT)