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Series GSE142545 Query DataSets for GSE142545
Status Public on Dec 22, 2022
Title Effects of deproteinized extract of calf blood on the energy metabolism of acute hypoxia model mice and its targets
Organism Mus musculus
Experiment type Expression profiling by array
Summary Deproteinized extract of calf blood (DECB) can improve the transport and utilization of blood oxygen and glucose in hypoxic tissue cells, and accelerate the oxidative phosphorylation process of mitochondria, to improve the energy metabolism of cells. Due to its complex composition, the mechanism of DECB on energy metabolism is not entirely clear yet. Effects of DECB on the energy metabolism of acute hypoxia model mice were observed by detecting glucose, lactic acid, and protein and ATP contents in the brain, heart and liver of the mice. Differentially expressed genes related to the effect of DECB on the energy metabolism of acute hypoxia model mice were screened with the mRNA chip and the correlation analysis was performed. Hmgcs2, Cpt1a, Angptl4, Cyp8b1 and Ehhadh genes were involved in the energy metabolism in mice, and mainly closely related to some biological processes, including ABC transporters, adipocytokine signaling pathway and PPAR signaling pathway. DECB can improve the body energy metabolism in mice, of which Angptl4, Cyp8b1 and Ehhadh are important factors in regulating pathways of glucose metabolism, Hmgcs2 gene can directly provide the energy in the lack of energy in the body, and Cpt1a may regulate the inflammatory reaction and promote the energy metabolism.
 
Overall design Male ICR mice, weighing 18-22g, were randomly divided into two groups, namely control group (CON) and DECB group, and 12 mice in each group. All the mice were administered continuously for 5 weeks and their body weights were weighed once every three days. Mice in the control group and DECB group were placed in containers with the same volume, the container mouth was evenly coated with a layer of vaseline, and all the container covers were closed at the same time while the timing started to ensure the start time of closing was the same. The asthmatic breathing accompanied by a slight convulsion of mice was taken as the indicator to immediately open the closed container, and then the hypoxia tolerance time of mice was observed and recorded.
 
Contributor(s) Xu G
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Submission date Dec 23, 2019
Last update date Dec 24, 2022
Contact name guangyu xu
E-mail(s) xuguangyu2005@163.com
Organization name College of Pharmacy, Beihua University
Street address Binjiang Road 3999,jilin city,132013
City jilin city
ZIP/Postal code +860432
Country China
 
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (6)
GSM4231698 Control-1
GSM4231699 Control-2
GSM4231700 Control-3
Relations
BioProject PRJNA597356

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE142545_RAW.tar 12.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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