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Series GSE142489 Query DataSets for GSE142489
Status Public on Jun 01, 2020
Title Single cell analysis of primary prostate human epithelial organoids grown in vehicle or 1,25D to day 8 and day 14
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Primary prostate epithelial cells derived from a single patient were grown as organoids in vehicle or 10nM 1,25D (vitamin D). At day 8 and day 14 of culture, organoids were dissociated to single cells, and collected & prepped for RNA-seq using the 10x Genomics Chromium Genome Reagent kit v3 Chemistry. ~5,000 cells were collected from each sample and sequenced at a depth of ~50,000 genes per cells on a 2x150nt lane in a NovaSeq 6000. CellRanger alignment was performed to produce the RAW data files.
Overall design Four samples: organoids grown to day 8 and day 14 in vehicle or 1,25D
Contributor(s) McCray T, Nonn L
Citation(s) 33458620
Submission date Dec 21, 2019
Last update date Jan 19, 2021
Contact name Larisa Nonn
Phone 3129960194
Organization name University of Illinois at Chicago
Department Pathology
Street address 840 S. Wood St, MC847
City Chicago
State/province IL
ZIP/Postal code 60612
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM4230367 V8
GSM4230368 D8
GSM4230369 V14
BioProject PRJNA597151
SRA SRP238486

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Supplementary file Size Download File type/resource
GSE142489_RAW.tar 332.7 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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