In order to investigate the dynamics of RNA secondary structure and changes in RBP-RNA interaction during mammalian erythropoeisis, we performed protein-interaction profile sequencing (PIP-seq) on MEL cells that are 0,2,or 4 days post differentiation
Overall design
2 replicates per timepoint of protein interaction profile sequencing (PIP-seq) in MEL cells. Each rep is crosslinked with formaldehyde and split into structure-only and protein-bound libraries and then further split based on RNAse treatment (i.e. ssRNAse or dsRNAse)
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