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Series GSE142116 Query DataSets for GSE142116
Status Public on Mar 31, 2020
Title 5' droplet-based single cell RNA sequencing of human colorectal cancer and normal organoids
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Using 5' droplet-based single cell sequencing, we profiled single cells dervied from human colorectal cancer organoids carrying either APC mutation or RSPO fusion, and paired normal colon organoids for the later.

 
Overall design Colorectal cancer and normal organoids were cultured in standard colon organoid medium or specified culture conditions to achieve differentiated state. Organoids were then FACS-sorted to obtain single cells and were subjected to sequencing.
The raw data files have not been included due to privacy concerns.
 
Contributor(s) Leung S
Citation(s) 32217638
Submission date Dec 16, 2019
Last update date Jun 30, 2020
Contact name Suet Yi Leung
E-mail(s) suetyi@hku.hk
Organization name The University of Hong Kong
Department Department of Pathology
Street address Queen Mary Hospital, Pokfulam Road
City Hong Kong
ZIP/Postal code Nil
Country Hong Kong
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (12)
GSM4221118 H002-TO
GSM4221119 H007-TO
GSM4221120 H009-TO
Relations
BioProject PRJNA595931

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE142116_RAW.tar 2.2 Gb (http)(custom) TAR (of TXT)
Processed data provided as supplementary file
Raw data not provided for this record

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